Project description
Visualisation of multi-drug efflux pump activity in living bacteria with nanophotonic biochips
Nanoengineered photonic chips hold great promise for bioimaging. Funded by the Marie Skłodowska-Curie Actions programme, the Illuminate-AMR project aims to improve imaging of membrane processes in cells with photonic biochips and study population dynamics of multi-drug efflux pump activity in antimicrobial-resistant bacteria via wide-field 3D nanoscopy. The objectives include development and testing of a new nanophotonic biochip with which to visualise and track membrane bound proteins in real-time under common light microscopes; development of a direct assay for imaging the presence and activity of multi-drug efflux pumps in bacterial membranes at the single-cell level; and comparison of the biochip platform with modern single-molecule imaging approaches.