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Single-molecule visualization of individual multi-drug efflux pump activity in living bacteria with nanophotonic biochips

Project description

Visualisation of multi-drug efflux pump activity in living bacteria with nanophotonic biochips

Nanoengineered photonic chips hold great promise for bioimaging. Funded by the Marie Skłodowska-Curie Actions programme, the Illuminate-AMR project aims to improve imaging of membrane processes in cells with photonic biochips and study population dynamics of multi-drug efflux pump activity in antimicrobial-resistant bacteria via wide-field 3D nanoscopy. The objectives include development and testing of a new nanophotonic biochip with which to visualise and track membrane bound proteins in real-time under common light microscopes; development of a direct assay for imaging the presence and activity of multi-drug efflux pumps in bacterial membranes at the single-cell level; and comparison of the biochip platform with modern single-molecule imaging approaches.

Coordinator

BUNDESANSTALT FUER MATERIALFORSCHUNG UND -PRUEFUNG
Net EU contribution
€ 174 806,40
Address
Unter Den Eichen 87
12205 Berlin
Germany
Activity type
Research Organisations
Non-EU contribution
€ 0,00