Periodic Reporting for period 1 - TexTOM (TexTOM - X-ray texture tomography as a tool to enable, multi-scale, in-situ imaging of the enthesis, a biological hinge between bone and tendon)
Período documentado: 2022-07-01 hasta 2024-12-31
Hierarchical structures are signature elements of many biological and technical materials. The orientational distribution of their crystalline constituents (the crystallographic texture) is important for their mechanical properties. Resolving the local structure and orientation spatially while keeping a large field of view is an unsolved problem. TexTOM will solve this by introducing texture tomography, a new 3D x-ray diffraction imaging method. It will enable to study the enthesis, the biological connection between tendon and bone, and by in-situ deformation and micromechanical modelling, couple its hierarchical structure with the mechanical behaviour.
By using the brilliance gain of 4th generation synchrotrons to develop texture tomography as a tool to image complex crystallographic textures in 3D, this project overcome the spatial resolution barriers of current approaches.
This project will develop the reconstruction approach for the 3D reconstruction of the crystallographic texture and use it to image the whole enthesis structure with 100nm spatial resolution and, with high energy x-rays, image the enthesis structure during in-situ tensile deformation with µm resolution at several load steps. The unique combination of 3D texture information and loading will allow to build a micromechanical enthesis model.
The novelty lies in the structural 3D characterization of the enthesis under deformation. This is enabled by the development of texture tomography to reconstruct the 3D textures, which will be useful for many other scientific problems.This will help to build an accurate micromechanical enthesis model and this will shed light on the unknown load transfer mechanism in the enthesis on the nano- and crystal level.
The flexible, open-source approach of TexTOM will ensure adaptation for new users and scientific problems. 4th generation synchrotrons will propel texture tomography to the forefront of (bio)materials science, revolutionizing our study of crystallographic textures.
By using the brilliance gain of 4th generation synchrotrons to develop texture tomography as a tool to image complex crystallographic textures in 3D, this project overcome the spatial resolution barriers of current approaches.
This project will develop the reconstruction approach for the 3D reconstruction of the crystallographic texture and use it to image the whole enthesis structure with 100nm spatial resolution and, with high energy x-rays, image the enthesis structure during in-situ tensile deformation with µm resolution at several load steps. The unique combination of 3D texture information and loading will allow to build a micromechanical enthesis model.
The novelty lies in the structural 3D characterization of the enthesis under deformation. This is enabled by the development of texture tomography to reconstruct the 3D textures, which will be useful for many other scientific problems.This will help to build an accurate micromechanical enthesis model and this will shed light on the unknown load transfer mechanism in the enthesis on the nano- and crystal level.
The flexible, open-source approach of TexTOM will ensure adaptation for new users and scientific problems. 4th generation synchrotrons will propel texture tomography to the forefront of (bio)materials science, revolutionizing our study of crystallographic textures.
In the following, we are going to give an overview of the main research and technological achievements with respect to the previously defined goals. These are getting further explained in the sections below.
The project has three major goals,
-1) the development of texture tomography as a tool study the crystallographic orientation in 3D with 100 nm spatial resolution
-2) Determine the multi-scale load transfer in the enthesis structure during in-situ loading with texture tomography
-3) Build a micro-mechanical model which allows to predict elastic properties and enthesis damage thresholds
In terms of scientific output, work has been carried out mostly towards goals 1) and 2) in accordance with the time planning.
With respect to goal 1), we have established a full mathematical description of the texture tomography problem, build a high-performance code to carry out the inversions. The publication describing the code has been submitted to peer-review, a pre-print has been submitted and the code will be released to the public upon acceptance of the manuscript via a gitlab repository. Linked to the code development, we have started to organize a training workshop, to be held in Marseille in February 2025. The aim of this workshop is to train users in the use of the TexTOM code.
In order to accurately benchmark the method, the development of a benchmark sample system for spatial and angular resolution has been started. We have chosen to develop a series of nanoparticle-filled polymers and introduce different textures during the thermal processing steps. First synchrotron experiments with the system have been carried out and the data treatment is ongoing.
The work on goal 2) are processing well too in collaboration with our project partners. The full characterization of 2D sections with our multi-modal imaging approach for nanostructural, crystalline and elemental composition has been carried out. These experiments establish a clear base-line for the 3D experiments. Together with the strong help of our project partners, we have developed protocols for the preparation of small 3D samples for the TexTOM characterization with high, 200 nm spatial resolution and first, succesful experiments have been carried out.
We have furthermore used Texture tomography and derived methods to address current problems in the biomineral community together with our collaborators, in particular the bone community.
We are currently progressing in the development of an in-situ loading device, suitable for 3D TexTOM and hope to carry out first experiments in the coming semester.
The project has three major goals,
-1) the development of texture tomography as a tool study the crystallographic orientation in 3D with 100 nm spatial resolution
-2) Determine the multi-scale load transfer in the enthesis structure during in-situ loading with texture tomography
-3) Build a micro-mechanical model which allows to predict elastic properties and enthesis damage thresholds
In terms of scientific output, work has been carried out mostly towards goals 1) and 2) in accordance with the time planning.
With respect to goal 1), we have established a full mathematical description of the texture tomography problem, build a high-performance code to carry out the inversions. The publication describing the code has been submitted to peer-review, a pre-print has been submitted and the code will be released to the public upon acceptance of the manuscript via a gitlab repository. Linked to the code development, we have started to organize a training workshop, to be held in Marseille in February 2025. The aim of this workshop is to train users in the use of the TexTOM code.
In order to accurately benchmark the method, the development of a benchmark sample system for spatial and angular resolution has been started. We have chosen to develop a series of nanoparticle-filled polymers and introduce different textures during the thermal processing steps. First synchrotron experiments with the system have been carried out and the data treatment is ongoing.
The work on goal 2) are processing well too in collaboration with our project partners. The full characterization of 2D sections with our multi-modal imaging approach for nanostructural, crystalline and elemental composition has been carried out. These experiments establish a clear base-line for the 3D experiments. Together with the strong help of our project partners, we have developed protocols for the preparation of small 3D samples for the TexTOM characterization with high, 200 nm spatial resolution and first, succesful experiments have been carried out.
We have furthermore used Texture tomography and derived methods to address current problems in the biomineral community together with our collaborators, in particular the bone community.
We are currently progressing in the development of an in-situ loading device, suitable for 3D TexTOM and hope to carry out first experiments in the coming semester.
The publication of the TexTOM code is a definitive breakthrough for the field. It will enable for the first time the fully quantitative 3D characterization of crystalline textures within a material. The current push to develop a resolution-estimate target is another innovative approach that will push the state of the art significantly, once implemented and properly evaluated.
We are currently working on the implementation of the method at synchrotron beamlines, develop documentation and organize a training workshop for users.
We have also received a strong interested from the material science community in our method and are developing dedicated steps to help that community with our methods.
We are currently working on the implementation of the method at synchrotron beamlines, develop documentation and organize a training workshop for users.
We have also received a strong interested from the material science community in our method and are developing dedicated steps to help that community with our methods.