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Uncovering the Diversity of Cell-Cell Interactions that Impact Cell Fates

Project description

Unveiling cell interactions during development

Neural crest cells are a temporary group of cells in the developing embryo that will generate different tissues. Neural crest cells migrate down different pathways where they encounter microenvironments believed to play a key role in cell fate determination. Funded by the European Research Council, the CELLMATES project is interested in delineating these cell–cell interactions at the transcriptional level. Through a novel approach, researchers will obtain transcriptomic information on the microenvironment and identify its functional role in cell fate decisions. Results will contribute to our broader understanding of cell interactions in space and time.

Objective

Multicellular life relies on coordinated physical interactions and communication between cells. In these interactions, cells exchange information that determines their fate, physiological state and behavior. The substantial transcriptional heterogeneity that is evident even among cells considered the same type, however, raises questions about exactly which temporal and spatial interactions regulate development and homeostasis. During development, for instance, multipotent neural crest cells migrate along different pathways in which they encounter microenvironments that are thought to instruct their migration and fate choices. To ultimately reveal how cell communication shapes cell identities and cellular decisions, we need to know the transcriptional state of a cell and that of its neighbors. Current technologies either sequence single cells at high transcriptional resolution or localize cells in their spatial context but without capturing their precise transcriptional configuration. To overcome these limitations, we will establish a novel approach that provides high-resolution transcriptomic information on cellular microenvironments. We will use this approach to uncover the interactions and communication codes that neural crest cells are exposed to along their developmental path. In addition, we will develop strategies to systematically identify cell-cell interactions likely to determine migration and cell fate decisions, and elucidate their functional role in the developing embryo. Our overall goal is to uncover how the diversity of cell interactions orchestrates the development of a complex cell lineage. This approach and the insights obtained here will provide a foundation for studying how cells interact in other systems such as human tissues, and how these interactions are altered in disease.

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(opens in new window) ERC-2021-STG

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Host institution

CHARITE - UNIVERSITAETSMEDIZIN BERLIN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 499 900,00
Address
Chariteplatz 1
10117 Berlin
Germany

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Region
Berlin Berlin Berlin
Activity type
Higher or Secondary Education Establishments
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Total cost

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€ 1 499 900,00

Beneficiaries (1)

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