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Hybridization derived novel patterns of gene expression

Project description

Altered gene expression following hybridisation: a potential path to novelty

Hybridisation, the crossing of two organisms from different species, can lead to expression of novel trait values or combinations. Hybridisation occurs in many species in nature, and thus likely plays an important role in evolution. Transgressive gene expression is the formation of transgressive (extreme) patterns of gene expression in hybrid populations relative to the patterns of gene expression in the parental species. These extreme gene expression phenotypes in hybrids could result from the breaking up of the co-inheritance of gene regulatory elements, but this remains to be tested. The EU-funded HybridExpress project will provide insights into the role of hybridisation in producing novel variation with a focus on transgressive gene expression using four independent hybrid lineages of the Italian sparrow.

Objective

Hybridization has been documented to the extent where its importance for generating evolutionary novelty can no longer be questioned. In spite of this, we lack an understanding of the evolution of regulation of gene expression in hybrids, and its role in hybridization derived novelty. Gene expression evolves in a stabilizing manner where cis(local)- and trans(distal) regulatory elements co-evolve. As hybridization breaks up co-inheritance of regulatory elements transgressive hybrid expression patterns, transcending the ranges of both parent species, may arise in spite of intermediate genome composition. I propose to leverage my finding of transgressive gene expression in a wild hybrid species and address how hybridization produces transgressive gene expression. This research will give insights into how hybridization can contribute to the arrival of the fittest, by producing novel variation that selection can act on.

Objectives:
1. To determine how regulation of gene expression in hybrids can produce evolutionary novelty
2. To unravel the roles of hybridization induced transposable element-releases, alterations of methylation and larger structural variants in hybrid specific gene expression
3. To uncover the role of gene expression in avian hybrid sterility.

I will use four independent hybrid lineages of the Italian sparrow and experimental F1 hybrids to achieve these ambitious goals. HybridExpress will i) Uncover how hybrid gene expression can transcend parental ranges, producing evolutionary novelty, ii) Contribute insights into how the variation selection acts on arises, iii) Increase our understanding of hybrid genotype-phenotype maps, and iv) Reveal the role of hybrid misexpression in reproductive isolation.

Interdisciplinary character: This proposal will make extensive use of genomic methods and hence overlaps to some degree with the LS2 panel, but as the question addressed is evolutionary I deem the proposal best suited for evaluation by the LS8 panel.

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2021-COG

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Host institution

LUNDS UNIVERSITET
Net EU contribution

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€ 2 000 000,00
Address
Paradisgatan 5c
22100 Lund
Sweden

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Region
Södra Sverige Sydsverige Skåne län
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 2 000 000,00

Beneficiaries (1)

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