Global food security is increasingly under threat due to the dual pressures of climate change and rising demand. With the global population expected to reach 9.2 billion by 2050, experts estimate that food production will need to double to meet future needs. Historically, advances in traditional breeding techniques, mechanization, and irrigation have driven significant yield increases for major crops. However, climate change, particularly global warming, now poses a significant obstacle to further progress. The Intergovernmental Panel on Climate Change (IPCC) highlighted crop heat stress as a critical risk to global food supply in its fourth assessment report. Wheat, the third most-produced cereal and the top crop in global trade, has often struggled to meet market demand, a challenge expected to intensify as demand rises by 60% by 2050. Heat stress is a significant limitation to wheat productivity, necessitating innovative solutions. Addressing this issue requires a deeper understanding of the molecular mechanisms governing heat stress and the development of tools to produce heat-tolerant varieties of bread wheat (Triticum aestivum). Over recent decades, wheat breeding has benefited from identifying favorable genetic variations, driven by advances such as sequencing the wheat genome, studying related species, and employing Quantitative Trait Loci (QTL) mapping and Genome-Wide Association Studies (GWAS). In the post-genomic era, exploring regulatory networks that control heat stress has emerged as a promising strategy. Epigenetic mechanisms, including histone modifications and DNA methylation—both reversible processes—play a crucial role in helping plants adapt to environmental challenges. While significant progress has been made in understanding these processes in model plants like Arabidopsis thaliana, applying this knowledge to crops with larger and more complex genomes, such as wheat, remains a relatively underexplored frontier. The 3Dwheat project seeks to advance our understanding of how epigenetic regulation influences heat stress resilience in wheat. By integrating multiple layers of regulation, rather than focusing solely on specific epigenetic markers, the project aims to develop a comprehensive toolkit. This approach holds potential not only for fundamental research but also for addressing practical challenges in wheat breeding, ultimately enhancing crop productivity in the face of a changing climate.
