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Vortex enhanced liquid chromatography for HbA1c monitoring based diabetes management in a globalized setting

Objective

Diabetes is one of the main health risks today with near pandemic dimension, causing blindness, kidney failures, stroke, heart attack, giving rise to very high health care costs (25% in the US) and reducing the quality of life of around 500 million people worldwide. The level of hemoglobin A1c (HbA1c) is used to assess long-term glycemic control and is the best predictor for the risk of developing chronic complication of diabetes and appropriate follow up of the patients. The golden standard is an expert laboratory HPLC method focusing on HbA1c quantification, which has limitations when other relevant hemoglobin variants are to be detected. For approximately 7 % of the world’s population which are carriers of such hemoglobin variants current methods lead to under-, over- or non-estimation of the HbA1c fraction. VortexLC will not only improve the quality of the analysis to give an instant full picture of the health status of diabetes patients, it will also produce a cheap point of care device. The use of vortex flows renders the approach compatible with mass manufacturing of plastic pillar array columns, that are not only much cheaper than commonly used packed bed columns, but wherein also higher separation performances can be obtained. The polymer columns will be fabricated using UV-nanoimprint lithography, plasma technology to make them porous and add a chromatographic coating, and lamination to close the column, all processes that can be scaled to roll-to-roll industrial manufacturing. The columns will be embedded in an instrument that allows for integrated sample preparation and miniaturized UV absorption and SERS detection, allowing for both quantification and identification of analytes. In the project a low footprint demonstrator of the novel system and columns will be built and tested with first synthetic, next human blood samples to quantify Hb1Ac and its genetic variants.

Coordinator

VRIJE UNIVERSITEIT BRUSSEL
Net EU contribution
€ 1 196 067,50
Address
Pleinlaan 2
1050 Brussel
Belgium

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Region
Région de Bruxelles-Capitale/Brussels Hoofdstedelijk Gewest Région de Bruxelles-Capitale/ Brussels Hoofdstedelijk Gewest Arr. de Bruxelles-Capitale/Arr. Brussel-Hoofdstad
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00

Participants (3)

"NATIONAL CENTER FOR SCIENTIFIC RESEARCH ""DEMOKRITOS"""
Greece
Net EU contribution
€ 555 000,00
Address
End Of Patriarchou Grigoriou E And 27 Neapoleos Street
15341 Agia Paraskevi

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Region
Βόρειος Τομέας Αθηνών Aττική Αττική
Activity type
Research Organisations
Non-EU contribution
€ 0,00
JOANNEUM RESEARCH FORSCHUNGSGESELLSCHAFT MBH
Austria
Net EU contribution
€ 708 108,00
Address
Leonhardstrasse 59
8010 Graz

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Region
Südösterreich Steiermark Graz
Activity type
Research Organisations
Non-EU contribution
€ 0,25
COATING PLASMA INNOVATION SAS
France
Net EU contribution
€ 555 200,00
Address
59 4 Eme Rue Zac Saint Charles
13710 Fuveau

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Region
Provence-Alpes-Côte d’Azur Provence-Alpes-Côte d’Azur Bouches-du-Rhône
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00