Descripción del proyecto
Microscopía tomográfica para el análisis histopatológico intraoperatorio
La histología, la evaluación de los tejidos y las células mediante un microscopio, resulta fundamental para el diagnóstico, la estadificación y la dirección del tratamiento del cáncer. Asimismo, requiere la extracción de biopsias, que se seccionan y tiñen para su posterior investigación. Sin embargo, el protocolo de tinción no es aplicable «in vivo», y la inspección final de la muestra de tejido solo es posible tras varias horas. El proyecto SpeckleCARS, financiado con financiado con fondos europeos, tiene como objetivo desarrollar una microscopía tomográfica rápida y sin marcadores con sensibilidad vibratoria para obtener imágenes en tridimensionales con una calidad comparable a las muestras teñidas tradicionales. El uso de un esquema de reflexión de campo amplio y el contraste sin marcadores no requieren la eliminación de tejido, además de proporcionar datos histológicos y tridimensionales intraoperatorios al instante.
Objetivo
Histology is central to the diagnosis, staging, and treatment of cancers. It requires the removal of small regions of suspect tissues (biopsies) that are later sectioned and stained with haematoxylin and eosin (HE). However, histology suffers from major weaknesses: First the standard HE protocol cannot work in vivo and requires ex vivo biopsies. Second HE is labour intensive, time consuming and the final tissue sections inspection is possible only after 12 hours. A faster but less accurate protocol, known as extemporaneous histology, can be performed in 40 min and is used in an intra-operative context to guide surgeries. The SpeckleCARS project aims to develop for the first time fast label free tomography microscopy with vibrational sensitivity to provide images with tridimensional improvement as compared to extemporaneous histology and in near-perfect concordance with conventional stained HE histology. This pioneering approach overcomes all the previous challenges: Using a wide field reflection scheme and label free contrast rules out all the drawbacks due to tissue removal and external labelling. It provides instantaneous intraoperative 3D histological data, improving the accessibility and accuracy of tumour detection, diagnosis and treatment. Combining the latest advances in 3D tomography reconstruction algorithms, high power laser systems together with key innovations in random speckle illumination and hyperspectral vibrational imaging, the SpeckleCARS project will provide for the first time volumetric histology imaging in real time over large (mm) fields of view without the need of tissue removal. To demonstrate the power of the developed technologies both ex vivo human and in vivo mouse cancer assessments will be conducted in collaboration with pathologists. The SpeckleCARS approach is broadly applicable to all cancer types and to any tissue diagnostic requiring histological data, so the project breakthroughs will benefit many medical and biology applications.
Ámbito científico
Programa(s)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Régimen de financiación
ERC - Support for frontier research (ERC)Institución de acogida
75794 Paris
Francia