Development of a revised plant synthetic biology toolkit to advance crop improvement

Información del proyecto

REPLANT

Identificador del acuerdo de subvención: 101060616

DOI

10.3030/101060616

Proyecto finalizado el 31 Diciembre 2022

Fecha de la firma de la CE 17 Mayo 2022

Fecha de inicio 1 Junio 2022

Fecha de finalización 30 Noviembre 2024

Financiado con arreglo a

• Marie Skłodowska-Curie Actions (MSCA)

Coste total

Sin datos

Aportación de la UE

€ 206 641,20

Coordinado por

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain

Periodic Reporting for period 1 - REPLANT (Development of a revised plant synthetic biology toolkit to advance crop improvement)

Período documentado: 2022-06-01 hasta 2024-11-30

Resumen del contexto y de los objetivos generales del proyecto

The aim of REPLANT was to generate a new plant SynBio toolkit based on the Phytobrick architecture that included the latest developments in protein production and gene editing techniques (Work Package 1). The advantages provided by the REPLANT were going to be demonstrated by two proof-of-concept experiments with biotechnological applications (Work Package 2): enhancing the production of SARS-CoV2 recombinant antibodies in plant leaves, and modification of tomato plant architecture in variants of commercial interest. This toolkit, alongside its dissemination approaches would allow a faster advancement of plant SynBio, accelerating the development of improved crops to help solve the global challenges of sustainability and food security.
REPLANT was planned as a two-year project with a six-month secondment in the multinational seed company Semillas Fitó. However, REPLANT was terminated after seven months because Eduardo González Grandío obtained a Ramón y Cajal Contract from the Spanish State Agency for Research that was not compatible with the MSCA Postdoctoral fellowship.

Trabajo realizado desde el comienzo del proyecto hasta el final del período abarcado por el informe y los principales resultados hasta la fecha

We generated a preliminary version of a Codon-optimization tool. We generated standarzied GoldenBraid parts and adapted the Prime Editing reagents to the Phytobrick standard. We generated tomato plants including the optimized Prime editing constructs