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In vitro reconstitution of the initiation of mitophagosome formation

Objective

Mitophagy is a tightly controlled process in which dysfunctional mitochondria are isolated and selectively degraded by autophagy. Many cell types critically rely on this pathway and mitophagy is therefore essential to life. For instance, egg fertilization or red blood cell development would not be possible without mitophagy. The importance of mitophagy is further underscored by its many links to human disease, most evident by mutations in the protein kinase PINK1 and the E3 ubiquitin ligase Parkin, causing autosomal recessive Parkinson’s disease. While most factors required for mitophagy are known, a major outstanding question is: how do the different factors come together to produce a mitophagosome? As the process comprises many different factors, each with a unique role, this is a challenging scientific question to address. The goal of this project is to gain insight into this process, using biochemical reconstitution, to elucidate how more than 15 different factors work together to initiate the formation of a mitophagosome. A unique aspect about our approach is that it not only allows to elucidate how mitophagosome formation is initiated, it could also reveal additional layers of fine-tuning, for instance also explaining how mitophagy is prevented from being initiated too early. This project may therefore lead to a major step forward, establishing a coherent framework of how mitophagosome formation is organized. Moreover, it could lead to new avenues for treating devastating neurodegenerative diseases like Parkinson.

Fields of science (EuroSciVoc)

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Keywords

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2021-PF-01

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Coordinator

UNIVERSITAT WIEN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 199 440,96
Address
UNIVERSITATSRING 1
1010 WIEN
Austria

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Region
Ostösterreich Wien Wien
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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