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CORDIS

Precise Genome Editing to Correct Cardiomyopathies in Human Cardiac Organoids

Project description

Gene editing therapy to treat cardiomyopathies

Inherited cardiomyopathies are a major cause of sudden cardiac death and are caused by mutations in genes encoding sarcomeric proteins in the heart muscle. There is no cure for cardiomyopathies so the EU-funded project Edit-hCOs aims to use CRISPR-Cas9 precision genome editing technology to correct the responsible mutations. The researchers have developed human cardiac organoids with cardiomyopathy-causing mutations to test the efficacy of the gene editors and rescue the disease effects. The project will also generate humanised mouse models harbouring the same mutations as patients to study safety and efficacy of the gene treatments for permanent treatment of cardiomyopathies.

Objective

Inherited cardiomyopathies are a major cause of sudden cardiac death and are caused by mutations in genes encoding sarcomeric proteins. With an incidence of 3 per 1,000 adults within the European population, inherited cardiomyopathies present a substantial burden to the healthcare services of all European nations. Although a few medications can slow the progression of the disease, today there is no cure for cardiomyopathies. The CRISPR-Cas9 precision genome editing technologies, base and prime editing, can permanently edit point mutations of genes, making them ideal tools to treat cardiomyopathies. To demonstrate the efficacies and therapeutic benefits of these editors in correcting cardiomyopathy-causing mutations, it is necessary to develop robust in vitro models of mature human cardiomyocytes that recapitulate the cellular complexity of the human heart. In the Edit-hCOs project, I will generate and characterize human cardiac organoids (hCOs) harboring clinically relevant cardiomyopathy-causing mutations in the FLNC gene, which encodes for the sarcomeric protein filamin C. hCOs will be generated as three-dimensional scaffold-free cardiac microtissues using tri-cellular combinations of human induced pluripotent stem cell -derived cardiomyocytes, cardiac fibroblasts, and cardiac endothelial cells. Then, I will deploy base and prime editors to fix common FLNC gene mutations in the hCOs, and I will characterize their molecular and functional improvements following correction. In addition, I will generate humanized mouse models harboring the same mutations as patients for future studies on delivery, therapeutic safety, and efficacy of the genome editing components in vivo. Completion of the Edit-hCOs project will allow me to establish a completely innovative and ambitious research line focused on the permanent treatment of cardiomyopathies. This will establish a crucial and important pre-clinical step towards therapeutic genome editing of cardiovascular diseases.

Funding Scheme

MSCA-PF - MSCA-PF

Coordinator

UNIVERSITA DEGLI STUDI DI PADOVA
Net EU contribution
€ 188 590,08
Address
VIA 8 FEBBRAIO 2
35122 Padova
Italy

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Region
Nord-Est Veneto Padova
Activity type
Higher or Secondary Education Establishments
Links
Total cost
No data

Partners (1)