Project description DEENESFRITPL Innovative platform for detecting early breast cancer biomarkers in exosomes extracted from blood Early detection in the case of breast cancer dramatically increases the survival rate. Exosomes are particles secreted from the cells into the body fluids. They emerged as biomarkers for liquid biopsy, carrying information about the cell of origin. Funded by the Marie Skłodowska-Curie Actions programme, the EXCEED project aims to develop a platform for the isolation and label-free optical characterisation of exosomes extracted from blood. The platform will combine the label-free imaging in interferometric microscopy and the chemical content information obtained by Raman spectroscopy. It will be tested using the exosomes from tumour cells to determine possible biomarkers for diagnosis. The final platform validation will be performed on healthy donors and breast cancer patients. Show the project objective Hide the project objective Objective Breast cancer is the most common cancer among women worldwide. Early detection is the most critical element, as the survival rate increases by up to 99%. Although there are conventional screening techniques such as mammography, breast cancer is still the second leading cause of cancer death in women. One of the holy grails in cancer research is to develop a blood test for early detection. Sensitive, non-invasive, and easy-to-use liquid biopsy techniques will revolutionize cancer diagnosis and treatment monitoring. The goal of this project is to develop a complete platform for the isolation and label-free optical characterization of biological nanoparticles called exosomes extracted from blood. Exosomes are secreted from the cells into the body fluids and play an important role in intercellular communication. These particles are emerged as potential biomarkers for liquid biopsy applications, as they carry information about their original cell. However, the translation of exosome-based analyses from research labs to clinical settings is limited due to the lack of efficient isolation and quantification tools available. We aim to develop a novel exosome characterization platform, named multimodal Interferometric-Raman imaging platform to detect and quantify exosomes at a single-particle level without using any labels. The combination of the label-free imaging capability of interferometric microscopy and the chemical content information provided by Raman spectroscopy will be an important step for exosome-based diagnostics applications. The proposed system will be integrated into a state-of-the-art isolation tool, creating complete isolation and multiparameter characterization platform. The proposed platform will be tested with the exosomes isolated from tumor cells to determine possible biomarkers that can be used for diagnosis. The diagnostic capability of the system will be validated with healthy donors and breast cancer patients(30 persons each) Fields of science natural sciencesbiological sciencescell biologynatural sciencesphysical sciencesopticsmicroscopymedical and health sciencesclinical medicineoncologybreast cancerengineering and technologymedical engineeringmedical laboratory technologylaboratory samples analysisnatural sciencesphysical sciencesopticsspectroscopy Keywords Biosensing Exosomes Liquid Biopsy Microfluidics Programme(s) HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme Topic(s) HORIZON-MSCA-2021-PF-01-01 - MSCA Postdoctoral Fellowships 2021 Call for proposal HORIZON-MSCA-2021-PF-01 See other projects for this call Funding Scheme MSCA-PF - MSCA-PF Coordinator KOC UNIVERSITY Net EU contribution € 268 803,36 Address Rumeli feneri yolu sariyer 34450 Istanbul Türkiye See on map Region İstanbul İstanbul İstanbul Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window EU contribution No data Partners (1) Sort alphabetically Sort by Net EU contribution Expand all Collapse all BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY United States Net EU contribution € 0,00 Address Serra mall 450 94305 2004 Stanford See on map Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding No data
