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Catalytic Hairpin Assembly-based Point of Need Detection Device for Noroviruses

Project description

More convenient detection of Norovirus

Noroviruses (NoV) are the most common causes of foodborne disease. Infection causes acute gastroenteritis. During an outbreak, rapid diagnostic testing is essential to screen and manage patients. In this context, the CHAMPION project funded under Marie Skłodowska-Curie Actions will develop a high-specificity and -sensitivity diagnostic kit that is easy to use, cheap and convenient. While current tests are designed for use in well-equipped laboratories by trained technicians, CHAMPION’s test can be administered anywhere. The test has a rapid and non-enzymatic nucleic acid amplification system employing catalytic hairpin assembly reaction coupled with a paper strip that can detect NoV from food matrices.

Objective

CHAMPION aims to develop a rapid, point-of-need (PON) diagnostic kit for the detection of Noroviruses (NoV), one of the leading causes of foodborne infectious disease outbreaks. The main motivations to perform this work is to provide an easy-to-use, economically convenient and standardized NoV diagnostic kit with high specificity and sensitivity Currently, the most effective protocol is the real time PCR (RT-PCR) for the detection of NoV from food matrices. However, RT-PCR assays are designed for use in well-equipped laboratories with sophisticated laboratory infrastructure and highly trained technicians, and are unsuitable for use in limited infrastructure of or in the field. The CHAMPION aims to facilitates and development of, rapid and non-enzymatic nucleic acid amplification system employing catalytic hairpin assembly (CHA) reaction coupled with a paper strip that can detect NoV from food matrices. Thus, the CHAMPION incorporates the pre-concentration, CHA signal amplification, specific detection and signal visualization in a single miniaturized device. Thus, it can be anticipated that the long-term outcomes of the proposed work will deliver a novel, accurate, rapid and reliable diagnostic assay for the detection of NoV.

Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) HORIZON-MSCA-2021-PF-01

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Coordinator

INTERNATIONAL IBERIAN NANOTECHNOLOGY LABORATORY
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 172 618,56
Address
AVENIDA MESTRE JOSE VEIGA
4715-330 Braga
Portugal

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Region
Continente Norte Cávado
Activity type
Research Organisations
Links
Total cost

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