Project description DEENESFRITPL Charting brain signalling with shadows The brain is a complex organ which remains poorly understood. The EU-funded NeuroExcell project will utilise cutting-edge super-resolution shadow imaging techniques to minutely map the structure of an entire rodent brain. Using advanced fluorescence and computer modelling, cellular structures and the contents of extracellular spaces will be revealed as shadows. Researchers will also examine live tissue slices to glean clues as to how these structures and proteins in extracellular matrices orchestrate brain signalling. This will provide a never-before-seen view into how our own brains may function and will prove invaluable to the fields of neuroscience and cell biology. Show the project objective Hide the project objective Objective NeuroExcell aims to reveal physiological roles of the ECS structure and dynamics in directly regulating neuronal signalling, by taking advantage of the innovative new Super-resolution Shadow Imaging (SUSHI) technique. It will elucidate the nanoscale structure of the ECS of entire mouse brain hemispheres at different planes of the brain. Inherently, it will reveal simultaneously all cells as analyzable shadows what will provide unprecedented ECS maps, to complement general existing cellular maps. The results will further clarify the distribution and duration of structural ECS dynamics across areas, and will disclose the relation between ECS structure, dynamics and protein distribution in the extracellular matrix. Investigating this in live tissue slices will further allow direct visualization and analysis of diffusional processes what will help to disclose the physiological roles of the ECS.The fundamental nature of the results, and the advanced technological framework, will be of fundamental interest for neurophysiologists, glia cell biologists, and glymphatic system researchers, among others. NeuroExcell will be carried out in a multidisciplinary environment involving advanced fluorescence imaging methods, classic electrophysiological techniques, computational modelling, biophysical theory and biochemistry. For the project I will join an emerging neuroscience institute where I will further enhance and diversify my professional competences through advanced training and quality research, adding to my development toward becoming an independent research group leader. Fields of science natural sciencesbiological sciencesneurobiologynatural sciencesphysical sciencesopticsmicroscopysuper resolution microscopynatural sciencesbiological sciencesbiochemistrybiomoleculesproteins Keywords Brain extracellular space neuronal signaling extrasynaptic signaling microglia STED super-resolution Optogenetics Programme(s) HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme Topic(s) HORIZON-MSCA-2021-PF-01-01 - MSCA Postdoctoral Fellowships 2021 Call for proposal HORIZON-MSCA-2021-PF-01 See other projects for this call Funding Scheme HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships Coordinator UNIVERSIDAD DEL PAIS VASCO/ EUSKAL HERRIKO UNIBERTSITATEA Net EU contribution € 181 152,96 Address Barrio sarriena s n 48940 Leioa Spain See on map Region Noreste País Vasco Bizkaia Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 Partners (1) Sort alphabetically Sort by Net EU contribution Expand all Collapse all ACHUCARRO BASQUE CENTER FOR NEUROSCIENCE FUNDAZIOA Spain Net EU contribution € 0,00 Address Parque cientifico de la universidad del pais vasco rriko unibertsitatea edificio sede plant a 3 48940 Leioa See on map Region Noreste País Vasco Bizkaia Activity type Research Organisations Links Contact the organisation Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00
