ELECTROCHEMICAL DNA-BASED SENSORS FOR IMMUNOTHERAPY MONITORING

Informations projet

IMMUNO-MONITORING

N° de convention de subvention: 101069250

DOI

10.3030/101069250

Projet clôturé

Date de signature de la CE 25 Mai 2022

Date de début 1 Juin 2022

Date de fin 30 Novembre 2023

Financé au titre de

• European Research Council (ERC)

Coût total

Aucune donnée

Contribution de l’UE

€ 150 000,00

Coordonné par

ISTITUTO NAZIONALE DI BIOSTRUTTURE E BIOSISTEMI
Italy

Periodic Reporting for period 1 - IMMUNO-MONITORING (ELECTROCHEMICAL DNA-BASED SENSORS FOR IMMUNOTHERAPY MONITORING)

Période du rapport: 2022-06-01 au 2023-11-30

Résumé du contexte et des objectifs généraux du projet

During the initial part of the ERC Consolidator Grant PROTOOLKITS we have successfully demonstrated a novel approach for antibodies detection. The platform uses antigen-conjugated synthetic DNA strands to achieve the rapid and inexpensive detection of target antibodies. The goal of this project was to evaluate the commercial potential of this platform for immunotherapy efficacy monitoring with particular focus on the detection and characterization of bispecific
antibodies (BsAb). More specifically, the goal of this project was to validate a new DNA-based technology for the detection and monitoring of BsAb. This was in fact considered as the application that best highlights the features of our platform (versatility and programmability).

Travail effectué depuis le début du projet jusqu’à la fin de la période considérée dans le rapport et principaux résultats atteints jusqu’à présent

In this project we focused on a specific model BsAb target, Anti EGFR/Anti Muc1 BsAb. This BsAb joins together the recognition elements of two of the most used antitumor recognition elements targeting EGFR and Muc1, respectively. This BsAb can thus bind and inactivate two receptors simultaneously and is designed as a possible thereapeutic antibody for breast cancer, colon cancer, lung cancer, etc. In our project we have succesfully demonstrated the detection of such model BsAb at low nanomolar concentrations. The system we developed can efficiently detect the target BsAb with high sensitivity, specificity (no significant activation with monospecific antibodies was observed), and good selectivity in complex sample matrices (plasma).

Progrès au-delà de l’état des connaissances et impact potentiel prévu (y compris l’impact socio-économique et les conséquences sociétales plus larges du projet jusqu’à présent)

The new detection system we have characterized during this project presents advantageous features in comparison with standard methods such as ELISA that make it suitable for point-of-care applications. Cost analysis of the production of the sensing kits and of new kits development (for new targets) is also highly advantageous making the technology a promising starting point for a start-up company. We are now considering initiating a more commercially-oriented activity based on these results.