Descripción del proyecto
Análisis innovador de datos proteómicos para la identificación exhaustiva de isoformas proteicas
Las isoformas proteicas, que son versiones diferentes de una proteína a partir de un único gen, contribuyen a la diversidad molecular de la vida. Sin embargo, faltan pruebas exhaustivas sobre las isoformas proteicas a nivel proteínico, ya que hasta el 80 % de los datos proteómicos quedan sin utilizar durante el análisis. Para superar este reto, el equipo del proyecto ORIGIN, financiado por el Consejo Europeo de Investigación, propone un nuevo método que se basa en el descubrimiento de las huellas dactilares multidimensionales deterministas (ORIGIN) que las isoformas proteicas generan en las mediciones proteómicas. El equipo identificará de manera sistemática isoformas proteicas al aprovechar una novedosa estrategia de análisis de datos proteómicos basada en la espectrometría de masas. Este método consiste en entrenar redes neuronales profundas para predecir las ORIGIN, que posteriormente se utilizan para identificar y cuantificar las isoformas proteicas.
Objetivo
Did you know that ~80% of all proteomic data is not utilized? Proteins play a vital role in all biological processes and organisms. We believe that different versions of a single gene product – protein isoforms – shape the molecular diversity of life. However, comprehensive evidence on protein-level is not available. Chromatography-coupled tandem mass spectrometry (LC-MS/MS) is the de-facto standard for measuring proteomes, but it is not good at identifying isoforms because at least 80% of the recorded information is never used. I argue that isoforms leave a deterministic multi-dimensional fingerprint (ORIGINs) representing their physicochemical properties in each proteomic measurement. Therefore, the central aim of this project is to discover and quantify protein isoforms systematically by a novel MS-based proteomics data analysis strategy. By tapping into the wealth of data the proteomics community has already amassed, I will train deep neural networks that allow the prediction of ORIGINs. Second, I will implement an innovative data analysis strategy that utilizes ORIGINs to identify and quantify isoforms. Third, I will demonstrate that ORIGINs can be used to substantially broaden our understanding of the molecular diversity of life by showcasing its application on four emerging and challenging questions in proteome research of varying biological and technical complexity. This will allow me to address a fundamental open question in biology: to which extent and prevalence isoforms are actually translated and what functional roles they might be associated with. ORIGINs will improve the sensitivity, biological resolution and accuracy at which proteins and their isoforms can be identified and quantified. Beyond this, the concept of ORIGINs can be applied to and improve any proteomics experiments, and thus holds the potential to revolutionize MS-based proteomics as a technology and elevate the whole field of protein-based research.
Ámbito científico
- natural sciencescomputer and information sciencesdata science
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomics
- natural scienceschemical sciencesanalytical chemistrymass spectrometry
- natural sciencescomputer and information sciencesartificial intelligencecomputational intelligence
Palabras clave
Programa(s)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Régimen de financiación
HORIZON-ERC - HORIZON ERC GrantsInstitución de acogida
80333 Muenchen
Alemania