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The Glucocorticoid Receptor in Aging and Circadian Endocrinology

Project description

Rejuvenating effects of diet

Stress triggers the release of glucocorticoid hormones, which are associated with cardiometabolic disorders. Although certain dietary interventions such as caloric restriction may assist metabolic function, they also increase glucocorticoid levels, so it is unclear whether their benefits are due to or in spite of this hormone. Funded by the European Research Council, the GRACE project will work on the hypothesis that the benefits of caloric restriction are due to higher glucocorticoid levels. The project proposes to study the molecular and physiological effects of increased hormone levels. It hopes to identify pathways and genes that can be targeted to promote longer, healthier lives.

Objective

The pandemic is stressful for many. In response to stress, glucocorticoids are released. They play essential roles as endogenous hormones and clinically as drugs. High levels are associated with cardiometabolic disorders and with aging. In contrast, diets like caloric restriction ameliorate metabolic dysfunction and prolong lifespan. These diets, however, also increase glucocorticoids. Now the open question is: What are the molecular and physiological effects of increased hormone levels, and are these diets beneficial because or in spite of elevated glucocorticoids?
We recently found that nutrition reprograms glucocorticoid responses independently of the hormone level and that diurnal glucocorticoid action controls rhythmic gene expression to regulate circulating glucose and triglycerides during day and night. I hypothesize that the benefits of caloric restriction are due to higher glucocorticoid amplitudes, and that their study will uncover transcriptional features prolonging healthspan.
I propose to functionally distinguish between diet-induced positive and stress-induced negative glucocorticoid responses. GRACE will identify diet-specific, ‘rejuvenating’ transcriptional complexes and target genes, versus detrimental pathways triggered by excess glucocorticoids such as stress. Glucocorticoid receptor targets unique to caloric restriction will be determined via ChIP- and RNA-seq in Aim 1. The functional impact of diurnal glucocorticoid release will be dissected with a constitutively active receptor allele in Aim 2. I propose to map active transcriptional regulomes in caloric restriction, in youth and old age, by ChIP-MS in Aim 3. I postulate that enhanced glucocorticoid activity at the right time of day may boost circadian rhythms and promote longevity.
Ultimately, applying omics to study the molecular mechanisms of stress hormones will identify pathways and genes amenable to pharmacological or nutritional intervention for longer, healthier lives.

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(opens in new window) ERC-2022-COG

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Host institution

TECHNISCHE UNIVERSITAET MUENCHEN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 652 883,75
Address
Arcisstrasse 21
80333 Muenchen
Germany

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Region
Bayern Oberbayern München, Kreisfreie Stadt
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 652 883,75

Beneficiaries (2)

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