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The role of plant microbiota in the evolution of fungal pathogens and their repertoires of secreted proteins

Project description

Effector protein interactions in plants

Fungal plant pathogens pose a threat to crop production by secreting effector proteins that interact with plant immune systems. Although many effector proteins have been identified, their specific functions remain unknown. These proteins may also play a role in manipulating host microbiota and are influenced by the unique microbial communities of different plant species. The ERC-funded FungalSecrets project will investigate 2 000 effector proteins from four pathogens infecting wild relatives of crop plants. It will predict protein structures, identify homology relationships, and explore interactions and co-evolution. Additionally, the project will assess the antimicrobial properties of these effectors and their impact on 400 bacterial strains from the host plants. The findings are expected to boost our understanding of fungal pathogen ecology.

Objective

FungalFungal plant pathogens have a devastating impact on crop production. Decades of research have addressed the molecular interaction of pathogens with plant immune responses and have established effector proteins as key molecules secreted by pathogens to interact with plant receptors. Studies of fungal pathogens have revealed a large diversity of predicted effector proteins expressed during plant colonization, yet the functional relevance of most remains unknown. I hypothesize that fungal pathogens secrete a diverse repertoire of effectors to manipulate host-associated microbiota and facilitate host invasion. As distinct plant species assemble distinct microbial communities, I hypothesize that effector evolution and host specialization of pathogens, in part, is driven by the host-associated microbiota. FungalSecrets, will combine bioinformatics, evolutionary analyses, molecular microbiology approaches and plant infection assays. Our research will focus on 2000 effectors and effector-like proteins produced by four pathogens infecting wild relatives of crop plants. We will predict the protein structures of effectors to identify new homology relationships and to make inference of protein interactions and co-evolution. Next, we will identify antimicrobial properties and determine the effect of each effector protein on the growth of 400 bacteria isolated from the host plants of the pathogens. To overcome challenges of heterologous protein expression we will use different expression systems. To obtain mechanistic insights into microbial interactions, we will conduct detailed analyses of a selected set of candidate effectors with pleiotropic functions in virulence and microbiota manipulation. FungalSecrets is based on innovative hypotheses and will cover a fundamental knowledge gap related to the ecology of fungal pathogens. The research will have implications for applied crop research and will stimulate new research of fungi associated with human and animal microbiota.

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Topic(s)

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2022-COG

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Host institution

CHRISTIAN-ALBRECHTS-UNIVERSITAET ZU KIEL
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 2 000 000,00
Address
OLSHAUSENSTRASSE 40
24118 Kiel
Germany

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Region
Schleswig-Holstein Schleswig-Holstein Kiel, Kreisfreie Stadt
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 2 000 000,00

Beneficiaries (1)

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