Periodic Reporting for period 1 - 3D-BrAIn (Revolutionary high-resolution human 3D brain organoid platform integrating AI-based analytics)
Période du rapport: 2023-04-01 au 2024-03-31
In this project we bring together three breakthrough technologies: 1) a novel, highly reproducible human brain modelling technology using robust adherent iPSC-derived 3D cortical organoid cultures, 2) a unique, state-of-the-art 3D multi-electrode array (MEA) technology for non-invasive high-resolution electrophysiological recordings and 3) a novel approach to analyze and interpret the large quantities of functional data using tailored automated machine learning (ML)-based algorithms.
With this approach we overcome significant hurdles that made it thus far impossible to create a truly representative and functional model of the CNS for personalized medicine, drug screening and neurotoxicity testing. The revolutionary 3D-BrAIn platform will allow robust and accurate modelling of the CNS for a broad range of neuropsychiatric diseases.
In this project a prototype of the 3D-BrAIn platform will be developed by growing functional 3D organoids that faithfully resemble the human cortex on 3D MEA micropillar electrodes, enabling continuous functional monitoring and by developing ML-based algorithms that can process and interpret the large spatiotemporal data sets. Once all individual components are optimized and integrated, proof-of-concept will be obtained by validating the platform using disease-relevant hiPSC cell lines.