HEDGE-A: High cell density engineering for the production of recombinant adeno-associated virus (rAAV).

Project Information

HEDGE-A

Grant agreement ID: 101105465

DOI

10.3030/101105465

Project closed

EC signature date 27 March 2023

Start date 1 May 2024

End date 30 April 2026

Funded under

• Marie Skłodowska-Curie Actions (MSCA)

Total cost

No data

EU contribution

€ 214 934,40

Coordinated by

DANMARKS TEKNISKE UNIVERSITET
Denmark

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Data Management Plan (1)

Data management Plan

Data management plan is also considers one training goal and will be delivered together with D1 (databases).

Publications

Peer reviewed articles (11)

ACS Synthetic Biology

Author(s): David Catalán-Tatjer; Saravana Kumar Ganesan; Iván Martínez-Monje; Lise M. Grav; Jesús Lavado-García; Lars K. Nielsen
Published in: ACS Synthetic Biology, 2024, ISSN 2161-5063
Publisher: ACS Publications
DOI: 10.1021/ACSSYNBIO.4C00382

Targeted UDP-glucose ceramide glucosyltransferase stable overexpression induces a metabolic switch improving cell performance at high cell density

Author(s): Marzia Rahimi, Lars K. Nielsen, Jesús Lavado-García
Published in: Frontiers in Bioengineering and Biotechnology, Issue 13, 2025, ISSN 2296-4185
Publisher: Frontiers Media SA
DOI: 10.3389/FBIOE.2025.1690203

Mixture design as a tool for improving full-to-empty particle ratios across various GOIs in rAAV production

Author(s): Konstantina Tzimou; Pol Hulsbus-Andreu; Ece Bahar Yildirim; Lars K. Nielsen; Jesús Lavado-García
Published in: Gene Therapy, 2025, ISSN 1476-5462
Publisher: Springer Nature
DOI: 10.1038/S41434-025-00546-5

Systematic insights into cell density-dependent transcriptional responses upon medium replacements

Author(s): Pérez-Rubio, Pol; Romero, Elianet Lorenzo; Cervera, Laura; Gòdia, Francesc; Nielsen, Lars Keld; Lavado-García, Jesús
Published in: Biomedicine & Pharmacotherapy, 2024, ISSN 1950-6007
Publisher: Elsevier
DOI: 10.1016/J.BIOPHA.2024.117640

Internalization of PEI-based complexes in transient transfection of HEK293 cells is triggered by coalescence of membrane heparan sulfate proteoglycans like Glypican-4

Author(s): Pol Pérez-Rubio, Meritxell Vendrell-Flotats, Elianet Lorenzo Romero, Kasper Enemark-Rasmussen, Laura Cervera, Francesc Gòdia, Jesús Lavado-García
Published in: Biomedicine & Pharmacotherapy, Issue 176, 2026, ISSN 0753-3322
Publisher: Elsevier BV
DOI: 10.1016/J.BIOPHA.2024.116893

Extracellular vesicle depletion and UGCG overexpression mitigate the cell density effect in HEK293 cell culture transfection

Author(s): Pol Pérez-Rubio, Jesús Lavado-García, Laia Bosch-Molist, Elianet Lorenzo Romero, Laura Cervera, Francesc Gòdia
Published in: Molecular Therapy - Methods & Clinical Development, Issue 32, 2024, ISSN 2329-0501
Publisher: Elsevier BV
DOI: 10.1016/J.OMTM.2024.101190

ACS Synthetic Biology

Author(s): Rahimi, Marzia; Adams, Anna Christina; Grav, Lise Marie; Nielsen, Lars K.; Lavado-Garcia, Jesus
Published in: ACS Synthetic Biology, 2025, ISSN 2161-5063
Publisher: ACS Publications
DOI: 10.1021/ACSSYNBIO.5C00861

Impact of specific productivity and operation mode upon the biophysical properties of HIV-1 Gag-based virus-like particles

Author(s): Pérez-Rubio, Pol; Lorenzo Romero, Elianet; Casas, Josefina; Díaz-Maneh, Andy; Gòdia, Francesc; Cervera, Laura; Lavado-García, Jesús
Published in: Applied Microbiology and Biotechnology, 2025, ISSN 1432-0614
Publisher: Springer Nature
DOI: 10.1007/S00253-025-13560-9

Proof-of-Concept for Cell Culture-Based Duck Fat Production on a Laboratory Scale

Author(s): Andy Díaz-Maneh; Andreu Camacho-Sucarrats; Javier Fuenmayor; Natasa Ilic; Natalia García-Aranda; Yuliana Enciso; Mario Notari; Francesc Gòdia; Jesús Lavado-García; Raquel Revilla-Sánchez
Published in: Food and Bioprocess Technology, 2026, ISSN 1935-5149
Publisher: Springer Nature
DOI: 10.1007/S11947-026-04259-4

Unlocking DOE potential by selecting the most appropriate design for rAAV optimization

Author(s): Konstantina Tzimou; David Catalán-Tatjer; Lars K. Nielsen; Jesús Lavado-García
Published in: Molecular Therapy - Methods & Clinical Development, 2024, ISSN 2329-0501
Publisher: Cell Press
DOI: 10.1101/2024.05.06.592659

Unravelling the essential elements for recombinant adeno-associated virus (rAAV) production in animal cell-based platforms

Author(s): David Catalán-Tatjer, Konstantina Tzimou, Lars K. Nielsen, Jesús Lavado-García
Published in: Biotechnology Advances, Issue 73, 2024, ISSN 0734-9750
Publisher: Elsevier BV
DOI: 10.1016/J.BIOTECHADV.2024.108370