Understanding the new oxidative paradigm of biomass waste upcycling

Información del proyecto

LyticPol

Identificador del acuerdo de subvención: 101106997

DOI

10.3030/101106997

Proyecto finalizado el 30 Septiembre 2025

Fecha de la firma de la CE 19 Julio 2023

Fecha de inicio 1 Abril 2024

Fecha de finalización 31 Marzo 2026

Financiado con arreglo a

• Marie Skłodowska-Curie Actions (MSCA)

Coste total

Sin datos

Aportación de la UE

€ 214 934,40

Coordinado por

SYDDANSK UNIVERSITET
Denmark

Periodic Reporting for period 1 - LyticPol (Understanding the new oxidative paradigm of biomass waste upcycling)

Período documentado: 2024-04-01 hasta 2026-03-31

Resumen del contexto y de los objetivos generales del proyecto

Tackling the climate crisis requires replacing fossil fuels with sustainable alternatives. An example of a renewable feedstock is lignocellulosic biomass. Its use has several advantages: since it is obtained, for example, from forest and agricultural residues, it does not compromise global food security, and it can be used to produce biofuels, as well as bioproducts, and more than 200 different chemicals. However, lignocellulose is a very recalcitrant material, hampering its use as a direct replacement for fossil fuels.
A promising way forward is the use of enzyme cocktails to break down lignocellulosic biomass. Important components of such cocktails are the enzymes lytic polysaccharide monooxygenases (LPMOs). To date, eight different LPMO families have been discovered. All eight families contain a single copper atom, which allows them to employ a unique oxidative mechanism to break down several polysaccharides. Yet, this mechanism remains elusive because too few LPMO families have been considered in previous studies, and the theoretical methods employed have been insufficient. The proposed “LyticPol” project aimed to develop a novel theoretical method and employ it on a large set of representative LPMO structures. The main scientific objective was to obtain a more general picture of the LPMO mechanism, as a basis for fine-tuning the industrial reaction conditions for LPMOs.
Overall, the project aimed to contribute decisively to the career goal of the researcher: to become an independent and innovative researcher in the field of bioinorganic chemistry.

Trabajo realizado desde el comienzo del proyecto hasta el final del período abarcado por el informe y los principales resultados hasta la fecha

The overall objective of decisively contributing to the researcher’s career goal has been fully achieved: the researcher accepted an assistant professor position with tenure track at a German university. Therefore, the project had to be terminated earlier.
Within the project duration, reaction mechanisms of two LPMO families have been studied, leading to the publication of two scientific articles and presentations of the results at three international conferences and two invited talks. Additionally, other mechanistic aspects that were not originally part of the proposal have been studied and will be published in three scientific articles; manuscripts are currently in preparation.

Avances que van más allá del estado de la técnica e impacto potencial esperado (incluida la repercusión socioeconómica y las implicaciones sociales más amplias del proyecto hasta la fecha)

The work performed led to new insights into the reaction mechanisms of copper enzymes degrading biomass. It demonstrated the importance of developing reliable computational methods and protocols to study the reaction mechanisms of copper enzymes. Further research will focus on gaining new insights into the diverse reaction mechanisms of LPMOs and related enzymes, as well as on developing protocols and methods to study them more reliably and extensively.