Description du projet
Nouvelles techniques d’imagerie pour la conservation des peintures anciennes
La préservation des peintures historiques nécessite un examen de leur composition chimique à l’échelle microscopique. La spectrométrie de masse à ions secondaires à temps de vol (ToF-SIMS) est une excellente technique pour cela, en raison de sa capacité à identifier à la fois les composants organiques et minéraux, associée à une résolution spatiale inégalée. Cependant, les ensembles de données résultant de ce processus d’imagerie ont tendance à être complexes. En outre, les peintures contiennent une combinaison de multiples éléments riches en protéines qui coexistent et subissent des processus de vieillissement. L’identification de la nature inhérente de ces éléments est un objectif majeur des experts en conservation. Dans ce contexte, le projet SCIMITAR financé par le programme MSCA s’attachera à créer une approche ToF-SIMS innovante, utilisant de nouvelles capacités d’imagerie par spectrométrie de masse en tandem et l’immunohistochimie basée sur le SIMS.
Objectif
Our cultural heritage is our shared past and should be preserved for future generations. In order to preserve an art object, it is not only essential to study its structure, creation, history, and significance, but it is also necessary to understand its past and current state as well as projected changes in the future. In the case of old paintings, this requires being able to correlate macroscopic observations with its chemical make-up at the microscopic level. Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) is a powerful chemical imaging technique, especially suited for heterogeneous systems such as paintings. ToF-SIMS can simultaneously localize and identify organic and mineral components and has the highest spatial resolution of all the molecular imaging mass spectrometry techniques. The sub-micrometer spatial resolution of ToF-SIMS is needed to investigate painting micro-samples that consist of sub-millimeter stacked layers of dried paint that contain both organic and inorganic ingredients. Case studies published over the last two decades attest to the potential of ToF-SIMS in providing information relevant for painting conservation. However, the resulting imaging datasets are very complex often causing a large proportion to remain unexploited. The high energy ion source of the instruments also causes larger biomolecules such as proteins to fragment, making it impossible to identify them. Meanwhile, a variety of protein-rich ingredients coexist and age in paintings, which means that identifying their nature would be extremely valuable to conservation experts. The proposal aims at tackling these weaknesses by developing methods to unambiguously identify the materials found in the heterogeneous microstructures of old paintings samples using a ToF-SIMS instrument with novel Tandem MS imaging capabilities as well as the development of a ToF-SIMS-based immunohistochemistry relying on metal-labeled antibodies to allow for targeted protein imaging.
Champ scientifique
Programme(s)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Régime de financement
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European FellowshipsCoordinateur
6200 MD Maastricht
Pays-Bas