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Exploring microbial community stability across mutational spectra

Objective

Interactions in which all partners benefit, known as mutualisms, underpin many vital ecosystem functions all across nature. Recent work by myself and others has shown that mutualisms can readily break down via a few independence-enabling mutations, especially under environmental stress. Understanding the mechanisms that ensure mutualism stability is of increased importance for environmental, health, and societal problems. An unexplored question is the impact that large variations in mutational rates and spectra can have on in this process. Indeed, mutator strains, variants with highly elevated and biased mutation rates, readily take over microbial populations when there is ample adaptive opportunity, such as under environmental stress. These dramatic changes in spontaneous mutagenesis can have opposing effects on mutualism stability. On the one hand, they can lead to adaptive mutations appearing more rapidly, promoting partners survival and hence stabilising mutualisms. On the other hand, they may accelerate the acquisition of independence-enabling mutations, hence destabilising mutualism. With MICROCOSMS, I will address this open question by combining computer simulation with experimental evolution and genomics. I will experimentally evolve pairwise auxotrophic E. coli communities, genetically engineered to contain mutator variants that alter the mutational spectrum available. Pairwise communities will be assessed for mutualism stability when subject to both adaptive evolution, under strong antibiotic pressure, and non-adaptive evolution, where populations are subject to severe bottlenecking. These will be matched to simulations predicting the outcome of adaptive and non-adaptive evolution. By producing key insights into the stability of mutualisms under environmental stress, MICROCOSMS will form a knowledge base that will aide in protecting environmental and human-based ecosystems against disruptions.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2022-PF-01

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Coordinator

UNIVERSIDAD POLITECNICA DE MADRID
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 181 152,96
Address
CALLE RAMIRO DE MAEZTU 7 EDIFICIO RECTORADO
28040 MADRID
Spain

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Region
Comunidad de Madrid Comunidad de Madrid Madrid
Activity type
Higher or Secondary Education Establishments
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Total cost

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Partners (1)

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