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Investigating microbial colonization and removal on dynamic patterned surfaces

Project description

Mechanical deformation: disrupting biofilms, controlling implant-related infections

Microorganisms like bacteria form communities encapsulated in an extracellular polymeric matrix that attaches to surfaces. These biofilms are very difficult to combat – the encapsulation forms a powerful barrier to antibiotics and chemicals. Whether on hospital surfaces, implants, or prosthetic devices, these biofilms can cause life-threatening infections. With the support of the Marie Skłodowska-Curie Actions programme, the MOBILE project will investigate the potential of mechanical deformation to cause detachment and surface cleaning. Specifically, using various ‘wrinkled’ surfaces and fluid shear, it will investigate effects on bacterial proliferation, motility, and viability. Then, it will deform the wrinkled topographies in the search for new ways of bacterial removal.

Objective

"Microbes have remarkable capabilities to attach to surfaces of natural and artificial systems, eventually leading to the formation of biofilms and associated chronic and persistent infections. It is extremely appealing to understand how bacteria interact with three- dimensional surface topographies and how to design smart patterns as a strategy to create antifouling and biocidal materials. Here I propose a dynamic strategy, merging verstile and large-scale surface modification teqhniques based on mechanical wrinkling of soft bilayers, that I developed at Imperial College London, microfluidics and microbiology. The goal of MOBILE is investigating the mechanical confinement exerted by non-planar surface curvatures and spatial heterogeneities induced by fluid shear on bacterial initial attachment and removal, in confined environments. Specifically (Aim 1), I will evaluate the combined action of surface topography and fluid shear over bacterial proliferation, motitly and viability, incorporating nano- to micro-scaled wrinkled geometries in microfluidic channels, mimicking biological tissues surfaces and implantable medical devices, testing a series of different clinically relevant bacterial strains (such as Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae). I will also (Aim 2) develop antifouling and removal strategies by investigating the mechanical response of adhered bacteria, using patterned surfaces as stimuli-responsive probes ""actuated"" by means of mechanical deformation (i.e. by extension and compression of the wrinkled topographies) to induce detachment and surface cleaning under fluid dynamic conditions. Overall, I aim to elucidate new methodologies for bacterial removal at different stages of biofilm formation paving the way towards the development of new classes of biomedical devices and to contribute to an important step in direction of controlling implant-associated infections."

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2022-PF-01

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Coordinator

UNIVERSITA HUMANITAS
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 172 750,08
Address
VIA RITA LEVI MONTALCINI 2/4
20072 PIEVE EMANUELE
Italy

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Region
Nord-Ovest Lombardia Milano
Activity type
Higher or Secondary Education Establishments
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Total cost

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