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A two-photon compound fiberscope to study the brain at all spatial and temporal scales.

Objective

Understanding how neuronal circuits process information is a major scientific challenge, which demands new tools to address the complexity of the brain in animals during natural functioning. Two-photon (2P) microscopy, combined with optogenetics, has revolutionized neuroscience thanks to the possibility to image and photostimulate neuronal activity with light, but suffers from important limitations.
To determine how perception and behaviour arise, we need to record and manipulate at will the activity of every neuron in a circuit in freely behaving animals. 2P microscopy is on the contrary mainly performed in head-fixed animals, which poses a clear limitation to the study of natural behaviour. At the same time, understanding how different brain areas exchange information requires to maintain single cell spatial resolution (~ 15 m) and temporal resolutions compatible with the propagation of neuronal signals (~ 1 ms) over ultra large spatial scales (5 mm), which is today completely out of reach for 2P microscopy.
In this project I will overcome these limitations and develop the 2P compound fiberscope, a new optical technique based on the unique combination of multiple optical fibers and optimal spatial and temporal beam shaping approaches, which will completely change the way we study neuronal circuits thanks to two main technologies. 1) A flexible 2P micro-endoscope to image and photostimulate neurons in freely moving animals, which will give access to entire brain regions with the highest imaging and manipulation efficiency and the fastest acquisition speed. 2) The first 2P mesoscope specifically conceived to image and manipulate neuronal activity with single cell resolution across the majority of the mouse cortex on temporal scales compatible with the propagation of neuronal signals. These technologies will pave the way for a real understanding of how neuronal circuits drive behaviour, and how different brain regions communicate to process neuronal information

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Programme(s)

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Topic(s)

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2023-STG

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Host institution

FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 666 199,62
Address
CARRER BALDIRI REIXAC PLANTA 2A 10-12
08028 Barcelona
Spain

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Region
Este Cataluña Barcelona
Activity type
Research Organisations
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 666 199,63

Beneficiaries (1)

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