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Trafficking mechanisms and physiological factors mediating a direct gut microbiota-brain neuron interaction

Project description

Trafficking mechanisms and sex-biased effects of gut microbial products on the brain

The microbiota-gut-brain axis, a bidirectional network linking the intestinal bacteria and the central nervous system, influences intestinal functions, mental health and beyond. Funded by the European Research Council, the MicrobiotaNeuroTalk project focuses on the direct effects of components of the peptidoglycan layer of bacterial cell walls known as muropeptides on brain neurons. This interdisciplinary research will investigate the transport mechanisms and the physiological factors, such as hormones, that influence the interactions of muropeptides with brain neuronal cells, shedding light on the regulation and sex-biased impacts of microbial products in the brain. Insights could revolutionise neurological disorder therapies that have a different impact on men and women and that are influenced by microbiota, such as Alzheimer's and Parkinson's diseases.

Objective

The gut-brain axis has emerged as a complex regulator of system-wide physiology, playing essential roles to maintain homeostasis, including contributions to brain development and activity, affecting host metabolism and behavior. The gut bacterial composition constantly fluctuates, allowing for the regular release of diverse microbe-derived compounds into the bloodstream. Although it is known that many gut-bacterial metabolites affect distant organs such as the brain, their direct interaction with brain neurons is rarely demonstrated. The impact of microbial metabolites on brain mechanisms are generally thought to be indirect due to interaction with, for example, the immune system or the vagus nerve. However, my previous work has shown that microbe-derived muropeptides reach the brain and decrease the spontaneous activity of brain neurons that express the Nod2 receptor. Remarkably, this direct interaction affected appetite and thermoregulation in a sex- and age-dependent fashion. Nevertheless, to further understand these direct interactions, some questions still need to be addressed: how does this compound reach the brain? Which factors may lead to this sex- and age-dependent neuronal activation? Are there other neuroactive bacterial compounds directly affecting brain neurons? Therefore, using interdisciplinary approaches, I propose to (1) unravel gut-brain trafficking mechanisms, (2) define physiological factors (e.g. hormones) that shape this microbe-neuron interaction and (3) describe new bacterial compounds that affect hypothalamic circuits and their downstream effects. This proposal will expose novel aspects of host-microbe interactions, leading to a more complete and integrated understanding of bacterial influence on host’s essential functions. It may also lead to new therapeutic approaches for neurological disorders that exhibit specific sex prevalence and where the microbiota is a factor in disease susceptibility, such as Alzheimer’s and Parkinson’s diseases.

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2023-STG

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Host institution

FUNDACAO GIMM - GULBENKIAN INSTITUTE FOR MOLECULAR MEDICINE
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 730 397,18
Address
AVENIDA PROFESSOR EGAS MONIZ
1649-035 LISBOA
Portugal

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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 730 397,18

Beneficiaries (2)

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