Molecular assays
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Design of molecular assays for the selected target genes, with details of assay optimization, detection limits and specificity testing against closely related non-target species. (Task 3.1 & 3.2)
Policy Brief 1
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Executive summary of developed technologies and intended use (Task 8.6)
Devices for organic contaminants
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Design and fabrication details of devices for aquatic contaminant preconcentration and immuno-SAW sensor, with description of operation and initial validation data from lab testing (Task 1.1, 1.2 &1.6)
Device for mRNA preconcentration
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Design and fabrication details of device for cell lysis and mRNA preconcentration. Description of operation and results of initial testing to select optimal materials and volumes. (Task 1.3)
Generation of diatom HM WCBs
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Generation of P. tricornutum and T. pseudonana Cd/Ni WCBs, including details of the assembly of the episomal vectors and generation of transgenic lines. (Task 5.2)
Real-time fluorescence analyzer
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Design and fabrication of device for real-time RNA amplification and multichannel fluorescence measurement unit. Description of operation using app and initial validation data from lab testing. (Task 1.4 & 1.6)
Diatom Cd/Ni responsive elements
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Details of experiments using Cd and Ni additions and transcriptomics, to identify Cd/Ni responsive promoters in P. tricornutum and T. pseudonana. (Task 5.1)
DEC plan and materials 1
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DEC plan and materials (Package 1) (Task 8.2)