The main achievement during this reporting period has been the development and transfer of the genetic fingerprint for rice lines, that lays the foundation for untargeted (i.e. whole sequence based) detection methods of NGT products. Several lines of NGT rice have been developed and fingerprinted in comparison to unedited lines. The methodology and approach have been worked into a scientific manuscript that was submitted in the reporting period. Expanding this approach to other species and other varieties is an important step for establishing surveillance, traceability and labeling of NGT products across the entire food chain.
In combination with a whole genome sequencing approach, DARWIN has established an enhanced PCR method that meets the required sensitivity and specificity. This method is in the process of being transferred to partners for a full ring trial.
Work package 1 is progressing according to plan. Fruitful co-creation workshops and collaboration with other relevant projects are in place. Stakeholder engagement and consumers interactions have also taken place. The untargeted methods in work package 2 are also progressing and yields exciting results at an early stage. The first scientific publication was submitted during the reporting period. DARWIN has experienced some delays in some methods in work package 2, and these are highlighted in this report. However, establishment of collaborating activities with DARWIN’s sister project, DETECTIVE was initiated, and has resulted in meeting activities and participation in workshops. We expect more collaborating activities to come in the coming reporting period. Transferability of the methods from work package 2 to 4 has partly been fulfilled, and a network of GMO laboratories have been identified to participate in the validation process of the methods. Interaction with work package 3 has started, but the output there is dependent on input from work package 2, which is a bit delayed. Nevertheless, efforts have been made to start development of the bioinformatics tools and support systems. DARWIN has had considerable activities tied to presentations, online exposure and publications directed at the general public.
