Project description
Elucidating collective behaviour and chemotaxis of Trypanosoma brucei
It is generally perceived that parasites act individually and that tissue specificity is driven by receptor-ligand interactions between pathogen and host surface molecules. Recently, however, the ERC-funded Tryptaxis project has discovered contrary behaviour in Trypanosoma brucei (responsible for sleeping sickness), which cycles between tsetse flies and mammals. This finding indicates that trypanosomes generate pH gradients on surfaces and use cyclic adenosine monophosphate signalling to move collectively in response to them. Essentially, they respond chemotactically to other metabolites and are not confined to the blood and central nervous system, but also invade and colonise other organs in host mammals. The aim of Tryptaxis is to thoroughly analyse the mechanisms that control directed migration in Trypanosoma brucei.
Objective
Many protozoan parasites have complex life cycles requiring migration through different organs in their hosts. The current mind-set is that parasites act as individuals and that tissue specificity is largely dictated by receptor-ligand interactions between pathogen and host surface molecules. However, parasites are more autonomous and manipulative than we give them credit for. I propose that self-steering, a mechanism by which groups of cells migrate in response to gradients that they create and/or modify, is central to their ability to orient themselves and home into host tissues.
Trypanosoma brucei, which causes sleeping sickness, cycles between mammals and tsetse flies. Trypanosomes lack G-protein coupled receptors (GPCR) and heterotrimeric G proteins, which act as sensors and signal transducers in yeast and multicellular eukaryotes, but encode a large number of receptor adenylate cyclases. We recently discovered that trypanosomes generate pH gradients on semi-solid surfaces and use cyclic AMP (cAMP) signalling to move collectively in response to them. This correlates with their ability to cross barriers and sequentially colonise organs in tsetse flies.
Our newest findings that trypanosomes respond chemotactically to other metabolites, together with evidence from several laboratories that T. brucei is not restricted to the blood and central nervous system in mammals, but also invades other organs, are the focus of this project.
My objectives are:
to elucidate the intracellular mechanism of cAMP-mediated pH sensing
to explore other cues for chemotaxis and determine how signals are transduced
to establish whether the expanded family of adenylate cyclase genes in T. brucei plays a role in extravasation and colonisation of different organs in mammalian hosts.
This is the first, comprehensive analysis of the mechanisms governing directed migration by a parasite and is a paradigm for new forms of sensing in eukaryotes lacking GPCR.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteins
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensors
- natural sciencesbiological scienceszoologymammalogy
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Keywords
Programme(s)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Topic(s)
Funding Scheme
HORIZON-ERC - HORIZON ERC GrantsHost institution
97070 Wuerzburg
Germany