Project description
Improved protein labelling supports studies of dynamics at distances
Proteins perform a plethora of functions in cells and tissues, many accomplished via conformational changes. Conversely, aberrant changes in protein structure are often linked to pathological conditions. Paramagnetic resonance enhancements (PRE) or single-molecule Förster resonance energy transfer (smFRET), two advanced spectroscopic techniques for studying real-time protein dynamics in solutions relying on site-specific labelling, have enabled measurements of interactions over different distances. Current labelling approaches dominantly rely on random cysteine labelling with only a handful of probes. With the support of the Marie Skłodowska-Curie Actions programme, the InProSpecT project aims to develop tailored, selective and easy-to-use fluorophores and labelling strategies for smFRET and PRE, allowing simultaneous studies of protein distances and dynamics.
Objective
Protein structures, including their complexes, determine their specific function and the molecular working mechanisms. The astonishing developments in X-ray crystallography and cryo-EM allow insights into atomic levels of protein structures, however, commonly on their static, solid state conformations. Nuclear magnetic resonance (NMR) and single molecule fluorescence spectroscopy, on the other hand, allow observations of protein dynamics in solution, in real-time. In particular, specific intermediate and long-range interactions can be measured using paramagnetic resonance enhancements (PRE) or single molecule Frster
Resonance Energy Transfer (smFRET), requiring site-specific labelling with paramagnetic or fluorescence probes, respectively.
Naturally, the choice of the label and its covalent attachment to the protein are critical, however, traditional approaches rely on random cysteine labelling with only a handful of available probes, making measurements difficult to interpret and laborious. As such, there is an urgent demand to develop custom-tailored, selective, orthogonal, easy to use chemical biology probes with excellent spectroscopic properties (for smFRET and PRE) to drive the field of protein structure and dynamics. This proposal tackles current limitations in the peptide/protein labelling field by creating state-of-the-art fluorophores for red/far-red smFRET. Building on this, the best performing dyes will be unleashed to defined labelling of intrinsically disordered and native proteins, in vitro and in cellulo, respectively. Next, these concepts will be expanded to be merged with intramolecular PRE probes to combine to date mutually exclusive spectroscopic properties. InProSpecT (Innovative Protein labelling strategies for Spectroscopic high resolution Techniques) pushes a bottom-up approach, from fluorophore design and labelling strategies ultimately linking two worlds to allow unprecedented, simultaneous studies of protein distances and dynamics.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences earth and related environmental sciences geology mineralogy crystallography
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics spectroscopy
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
-
HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
See all projects funded under this programme
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
See all projects funded under this funding scheme
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2023-PF-01
See all projects funded under this callCoordinator
Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
12489 Berlin
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.