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Transcription factors and regulation of AQP expression in maize stomatal guard cells and subsidiary cells

Objective

The stomata are openings formed on the epidermis formed by two guard cells (GC) surrounded by subsidiary cells (SC), allowing gaseous exchange for photosynthesis at the expense of water via transpiration. Stomata are tightly regulated by the influx and efflux of water and small solutes across GC or SC membranes. The diffusion of these solutes and water through the membranes can be facilitated by aquaporins (AQPs). While there is insufficient knowledge on the precise role of AQPs in stomata, specific microdissection of the mechanism of AQP action in GCs and SCs affecting stomata is further limited. The superiority of grass stomata owing to their quick response make them apt for studying the role of AQPs in stomatal response, especially under abiotic stress condition since climate change has made crops worldwide extremely vulnerable. In this regard, maize is an appropriate grass crop to use as a model because of its economic importance. The overall goal of this project is to study the role of different AQPs, and their transcriptional regulation in stomatal complexes, that can be used to produce stress-resilient crops. We will be using transactivation assays, protoplast engineering, maize suspension cells, and CRISPR/Cas to achieve the objectives. The mechanisms uncovered here can be monumental to extrapolate the grass stress resilience mechanisms into other vital crops. This project will help add the experience required for my goal of being an independent group leader. I will gain from the scientific environment and the infrastructure and support system at LIBST-UCLouvain as well as from the vast experience of Prof. Chaumont in research and project management. In turn, the host will benefit from the technical skills, and creativity that I can offer.

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Keywords

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

UNIVERSITE CATHOLIQUE DE LOUVAIN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 175 920,00
Address
PLACE DE L UNIVERSITE 1
1348 LOUVAIN LA NEUVE
Belgium

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Region
Région wallonne Prov. Brabant Wallon Arr. Nivelles
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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