Objective
Despite significant progress in chloroplast biotechnology the de novo synthesis of a chloroplast genome has
remained elusive. The development of strategies to design, assemble and transform artificial chloroplast genomes
would signify a step-change in the field of plant synthetic biology. Only recently, genome scale DNA synthesis
and sequencing has become routine, providing an ideal foundation to revisit the challenge of chloroplast genome
synthesis. I will join Ralph Bock, a world leader in chloroplast biotechnology, and combine the expertise of his
laboratory with my background in synthetic biology to generate the first Nicotiana tabacum plant with an artificial
chloroplast genome. I will base my strategy on creating and testing multiple different genome designs with varied
degrees of structural and genetic code alterations in respect to the parental genome to identify viable designs. I
will develop methods to assess those designs in parallel, permitting the rapid identification and fixing of
problematic regions in the synthetic genome. I will proceed to assemble the verified chloroplast genomes in a
hierarchical, parallelized manner from large pieces of synthetic DNA. Finally, I will characterize the phenotype of
the resulting plants, focusing on understanding the changes in transcription, translation and metabolism. The
methods developed in this work will provide insights into the design principles of organellar genomes, and provide
a platform to reinvent and remodel the genetic code of chloroplasts. This work will thereby establish strategies to
re-engineering complex cellular processes such as photosynthesis, or to design entirely new functions such as the
genetic isolation of synthetic DNA in plants.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences genetics genomes
- natural sciences biological sciences botany
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2023-PF-01
See all projects funded under this callCoordinator
Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
80539 MUNCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.