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Discovering a new family of hypermodified antimicrobial peptides from marine tunicates and exploring their potential applications

Project description

Unlocking the potential of antimicrobial peptides from marine tunicates

The increasing antimicrobial resistance of pathogenic bacteria and fungi is an urgent global healthcare issue that requires the creation of more effective antibiotics. Similarly, infections caused by invasive medical devices call for enhanced antimicrobial coatings. Antimicrobial peptides (AMPs) may provide the solution, but limitations currently exist in their cost, efficiency, stability and biotechnological production. With the support of the Marie Skłodowska-Curie Actions programme, the TuniPeps project will first analyse the biosynthesis and biochemical mechanisms of naturally occurring AMPs found in marine tunicates. It will then explore the possibility of enhancing the properties of these natural products and using them as blueprints to biotechnologically produce low-cost, safe and efficacious antimicrobial agents such as antibiotics and surface coatings.

Objective

The spread of antimicrobial resistance among human pathogenic bacteria and fungi is a major global and European concern, urgently necessitating the development of novel antibiotics. Another unsolved global healthcare problem is the burden of infections acquired through invasive medical devices. Antimicrobial peptides (AMPs) could be the solution to both problems, but they are currently limited by high cost, insufficient efficacy and stability as well as restricted chemical options for biotechnological production. However, in a group of marine animals: tunicates, there is a major, poorly characterized superfamily of AMPs with many advantageous natural chemical modifications. These unusual post-translational modifications (tryptophan bromination, arginine/lysine mono- and dihydroxylation, tyrosine hydroxylation, C-terminal amidation/oxidation) are predicted to improve potency and stability of the mature AMPs and might enable their efficient immobilization onto various surfaces. The latter property could also prove useful for surface coatings. In the current project, we aim to explore these promising bioproducts, from predictions by bioinformatics, through isolation of animal tissues, extraction and enrichment of the actual peptides, to their chemical analysis by mass spectrometry and testing of antimicrobial potency and safety. Project TuniPeps gives a major opportunity to both the applicant and host, to combine their complementary knowledge and skills in order to identify useful natural products from marine animals, acting as blueprints for future antimicrobials. These diverse molecules and their biosynthetic apparatus might also unlock cheap biotechnological production of novel molecules in the near future.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

UNIVERSITA DEGLI STUDI DI PADOVA
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 188 590,08
Address
VIA 8 FEBBRAIO 2
35122 PADOVA
Italy

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Region
Nord-Est Veneto Padova
Activity type
Higher or Secondary Education Establishments
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Total cost

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