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Detection of Chronic Toxicity of Xenobiotes using PANDORA-seq of small non-conding RNAs

Objective

The XENOSEQ project aims to develop tools for detecting epigenetic changes caused by chronic toxicity of chemicals by sequencing small non-coding RNA (sncRNA) profiles. Xenobiotics, including herbicides like glyphosate, have raised concerns regarding their possible effects on human and environmental health, but chronic and especially transgenerational effects are often ignored due to limitations of time and resources. This research project seeks to study whether the newly developed PANDORA-seq unveils subtle changes in sncRNA profiles that may serve as early indicators of chronic toxicity. PANDORA-seq utilizes two RNA modifying enzymes, T4 polynucleotide kinase and AlkB demethylase, to extend the repertoire of detected sncRNAs to an unprecedented world. Comprehensive sequencing of sncRNA profiles may reveal chronic toxicity associated with xenobiotic exposure. Here, we aim to 1) characterize baseline sncRNA profiles in healthy systems, offering a reference point for comparison; 2) expose biological systems to potentially toxic agents and track changes in sncRNA expression using PANDORA-seq; and 3) detect early indicators of chronic toxicity, allowing the development of a complementary tool for the assessment of chemical toxicity.
PANDORA-seq's precision and sensitivity make it a promising tool for studying epigenetic regulation, which may cause chronic and transgenerational diseases. As this MSCA project is a first effort to use sncRNA for chronic ecotoxicology, we open the research field by using the fruit fly as a model organism, but the technique can be easily adapted for studying other relevant organisms, such as honey bees and mammals.

Fields of science (EuroSciVoc)

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

JYVASKYLAN YLIOPISTO
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 215 534,40
Address
SEMINAARINKATU 15
40100 Jyvaskyla
Finland

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Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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