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Development of Nanobodies against Pseudomona aeruginosa and Staphylococcus aureus and evaluation as immunotherapeutics

Project description

Targeting bacterial infections in cystic fibrosis with nanobodies

Cystic fibrosis (CF) severely affects the lungs, specifically by causing thick mucus that traps bacteria and leads to persistent infections. These infections are difficult to treat and often worsen over time, causing significant health complications. With around 105 000 people worldwide suffering from CF, the disease dramatically impacts quality of life despite advances in treatment. Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA) are the main bacteria responsible for these chronic infections. Supported by the Marie Skłodowska-Curie Actions programme, the CF.Nbs.PA-SA project aims to develop new therapies using nanobodies (small, powerful proteins) that can target and neutralise SA and PA. This approach offers a promising solution to improve treatment and help CF patients live better, healthier lives.

Objective

Cystic fibrosis (CF) is a genetic disorder, impacting various organs due to CFTR gene mutations. Lungs are severely affected by secretions, harboring bacteria, causing recurrent, chronic, and often fatal infections in 90% of CF patients. Globally, around 105,000 people across 94 countries suffer from CF, with an improved median survival age of 30 to 40 years, yet poor quality of life persists due to daily treatment needs.
Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA) are the primary pathogens isolated from CF patients' respiratory tracts, leading to most CF-related deaths. Their factors of virulence contributes to extensive damage, and despite intensive treatments, infections persist, fostering antimicrobial resistance. Antibiotic resistance claims over 35,000 lives yearly in the EU alone, underscoring the urgent need for novel therapeutics to combat SA and PA infections.
Nanobodies (Nbs) offer revolutionary immunotherapy potential due to their unique attributes. They are ten times smaller than conventional antibodies, maintaining strong recognition capability at nanomolar levels. Nbs exhibit low immunogenicity, rapid tissue penetration, and can target previously inaccessible antigen sites. Their small size provides enhanced stability, thermal resistance, reversibility of unfolding, proteolytic resistance, and high solubility.
Given the prevalence and severe consequences of SA and PA infections, the central aim of the project is to develop therapeutic Nbs to mitigate the impact of these infections and improve the quality of life for CF patients.

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Topic(s)

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

CONSORCIO CENTRO DE INVESTIGACION BIOMEDICA EN RED M.P.
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 181 152,96
Address
CALLE MONFORTE DE LEMOS 5
28029 MADRID
Spain

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Region
Comunidad de Madrid Comunidad de Madrid Madrid
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Research Organisations
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