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Unraveling novel Archaeal Metabolic Pathways impacting Greenhouse Gas Emissions

Project description

Methane-producing microorganisms under the microscope

Climate change is a pressing issue, and understanding the sources of greenhouse gases is critical. Among these sources, methanogenic archaea (tiny microorganisms that produce methane) play a significant role. Although they are known to contribute to methane emissions, much about their metabolic processes remains unclear. In this context, the ERC-funded ARCHAEBOLIC project aims to uncover the hidden abilities of these archaea. Recent research has identified new ways these microorganisms can convert wood and other methylated compounds into methane and CO2. The project will focus on characterising these novel enzymes, exploring the physiology of the archaea and assessing their impact on the environment. By doing so, ARCHAEBOLIC hopes to improve our understanding of archaea and their contribution to greenhouse gas emissions.

Objective

Archaea are remarkable microorganisms that form next to bacteria and eukaryotes one of the three domains of life and have shaped Earth’s biogeochemistry and climate for billions of years. These microorganisms are the main producers of the greenhouse gas methane. Especially in view of climate change, it is crucial to understand all factors impacting greenhouse gas emissions. Methanogenic archaea are at the center of my research. Recent breakthroughs in this field have been driven by the discovery of many new archaeal lineages via (meta)genomic sequencing. However, physiological characterization and isolation attempts of archaea lag far behind and the evolution of methanogenic archaea is still heavily debated. Now is the right time to investigate the still underexplored metabolic capabilities of (methanogenic) archaea, their impact on greenhouse gas emissions and to enrich novel archaea.
I recently discovered a metabolic pathway enabling archaea to produce methane and/or CO2 from wood components and, in silico, from prevalent methylated compounds such as chloromethane. The novel enzyme systems involved are widespread and new metabolic pathways involving a variety of methylated substrates can be predicted, suggesting an important role of archaea in anaerobic conversions of lignin and various methylated compounds to methane and/or CO2.
Therefore, I aim to (i) characterize these novel enzyme systems biochemically and in regard to their evolution, (ii) study the physiology of the archaea using these systems and (iii) evaluate the role of these archaea in the environment and for methane and CO2 emissions.
This proposal has the potential to revolutionize our view on the metabolic versatility of archaea by unraveling novel mechanisms of methane and CO2 production in a ground-breaking manner. Identifying new archaeal substrates involved in methane and CO2 production enables us to assess the impact of these conversions on the environment and global methane and CO2 budgets.

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Host institution

PHILIPPS UNIVERSITAET MARBURG
Net EU contribution
€ 1 485 968,00
Address
BIEGENSTRASSE 10
35037 Marburg
Germany

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Region
Hessen Gießen Marburg-Biedenkopf
Activity type
Higher or Secondary Education Establishments
Links
Total cost
€ 1 485 968,00

Beneficiaries (1)