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The interplay of symbiont nutrient release and host phagosome maturation in photosymbiosis regulation

Project description

Heterotrophic hosts and algal endosymbiont interactions in photosymbiosis regulation

Mutualistic relationships between heterotrophic hosts and phototrophic algal endosymbionts significantly impact global nutrient cycles. However, global warming is increasingly disrupting these associations. Understanding this phenomenon requires resolving how these relationships are established and broken down. The ERC-funded PhagoPhoRe project will explore the idea that algal endosymbionts can avoid being digested by the host cell by releasing photosynthates and mimicking ongoing digestion processes. It will investigate the role of host phagosome maturation processes and the relationship between algal nutrient release and host phagosome arrest. By leveraging recent advancements in cell isolation and culture techniques, the project aims to gain insights into the processes involved in establishing, maintaining and breaking down the cnidarian-algal symbiosis.

Objective

Photosymbioses between heterotrophic hosts and phototrophic algal endosymbionts have repeatedly and independently evolved across the tree of life. The immense productivity of photosymbioses affects global nutrient cycles, yet global warming is disrupting these nutrient-exchange symbioses, destabilizing the ecosystems they support. Heat stress erodes the metabolic controls underlying the establishment and maintenance of mutualistic carbon recycling in these symbioses. A link between endosymbiont nutrient release and their intracellular maintenance could thus explain the evolutionary establishment as well as the ecological collapse of photosymbioses in the Anthropocene.

Harnessing recent advances in cell isolation and culture techniques, PhagoPhoRe seeks to understand the processes underlying the establishment, maintenance, and breakdown of the cnidarian-algal symbiosis. Specifically, I will investigate the idea that the release of photosynthates enables algal endosymbionts to evade phagosomal digestion and autophagic immune responses of the host by mimicking ongoing digestion processes. In three complementary work packages that combine state-of-the-art single-cell transcriptomics, proteomics, and isotopic imaging techniques, I will: 1) Characterize the role of host phagosome maturation processes in the intracellular establishment and maintenance of algal endosymbionts. 2) Investigate the postulated link between algal nutrient release and host phagosome arrest and immune suppression. 3) Examine the drivers of co-evolution towards obligate metabolic interactions in photosymbioses.

Deciphering the interplay between symbiotic nutrient cycling and host phagosome maturation will be key to understanding the establishment and subsequent breakdown of photosymbioses. These processes are unlikely restricted to photosymbioses and could reveal fundamental mechanisms enabling the establishment of endosymbioses and, as such, eukaryotic life itself.

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2024-STG

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Host institution

ALFRED-WEGENER-INSTITUT HELMHOLTZ-ZENTRUM FUR POLAR- UND MEERESFORSCHUNG
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 489 028,00
Address
AM HANDELSHAFEN 12
27570 BREMERHAVEN
Germany

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Region
Bremen Bremen Bremerhaven, Kreisfreie Stadt
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 489 028,00

Beneficiaries (1)

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