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Cross-kingdom symbionts: plant pathogens as insect mutualists.

Project description

Plant pathogens shed light on cross-kingdom symbiotic interactions

Herbivorous insects are key vectors of plant pathogens, facilitating their transmission to uninfected plants. However, how microbes balance their roles as plant pathogens and insect mutualists remains unclear, as does the genetic basis of pathogen specificity and host shifts between parasitism and mutualism. The ERC-funded SymPathy project aims to advance understanding of cross-kingdom symbiotic interactions and plant pathogen epidemiology by exploring the relationships between the fungal pathogen Fusarium, the leaf beetle Chelymorpha alternans, and their shared host plant, Ipomoea batatas. By integrating fieldwork, bioassays, and advanced technologies, such as sequencing, reverse genetics, and metabolomics, the project will investigate symbiont-driven defences, the genetic mechanisms underlying infections during host shifts, and the interactions between pathogens and their vectors.

Objective

By vectoring plant pathogens, herbivorous insects are key determinants in the epidemiology of numerous plant blights. For a vector-borne phytopathogen, natural selection favors upgrading insect fitness to enhance transmission to an uninfected plant. It is currently unclear how a microbe balances this dual symbiotic lifestyle, featuring markedly different interaction outcomes: as a plant pathogen and an insect mutualist. Likewise, little is known about the molecular mechanisms underlying pathogen specificity, and the genomic basis and consequences of cross-kingdom host shifts along the parasitism-to-mutualism continuum. I will tackle these questions using an emergent model system: the interaction between the fungal pathogen Fusarium, the leaf beetle Chelymorpha alternans, and their shared host plant Ipomoea batatas. I recently described that C. alternans associates with a fusarial strain that coats the pupae of its host, protecting beetles against predators during metamorphosis. In exchange, the insect propagates its symbiont to its host plant, where it causes wilt disease. The unique biology and tractability of this study system affords unparalleled opportunities for experimentation. My approach will combine fieldwork and bioassays, with the latest developments in sequencing technologies, reverse genetics and metabolomics to characterize (1) the mechanistic features of symbiont-driven defense, (2) the genetic basis of infection in a cross-kingdom host-shift, (3) the genomic consequences of a dual symbiotic lifestyle, and (4) the breadth, origin as well as partner fidelity and chemical stability of a pathogen-vector interaction. Bridging the fields of chemical ecology, plant pathology and natural product research, this project will significantly further our understanding of the genetic-basis of cross-kingdom symbiotic interactions and the epidemiology of plant pathogens.

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2024-STG

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Host institution

STICHTING VU
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 500 000,00
Address
DE BOELELAAN 1105
1081 HV Amsterdam
Netherlands

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Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 500 000,00

Beneficiaries (1)

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