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Understanding Long-term Trends in Multi-pathogen Epidemiology in Africa using Multiplex Serological and Molecular Assays

Project description

Multiplex serological assay for febrile illness treatment in Africa

Progress against malaria in sub-Saharan Africa is revealing a rise in non-malaria fevers. The disease burden is shifting from parasitic infections to viral infections, with a greater variety of pathogens emerging. The ERC-funded ULTIMASero project aims to investigate long-term trends in infectious diseases in Cameroon, Madagascar, and Senegal. It will develop a multiplex serological assay using Luminex technology to measure antibodies against over 300 pathogens. This analysis will include samples from febrile patients and 50 000 biobanked samples collected over three decades. The goal is to improve strategies for studying and treating febrile illness in Africa.

Objective

Enormous progress is being made in the fight against parasitic diseases such as malaria in sub-Saharan Africa, which is driving a long-term epidemiological shift in the burden of infectious diseases. Until recently, most febrile patients presenting to rural health facilities were presumptively treated for malaria. However, the decline in malaria cases coupled with the roll out of rapid diagnostic tests has revealed a substantial burden of non-malaria fevers. I hypothesise that the predominant disease burden in Africa is changing from parasites to viruses, and that the diversity of infecting pathogens is increasing over time.

ULTIMASero aims to investigate long-term trends in the epidemiology of infectious diseases by taking a multi-pathogen approach using multiplex serological and molecular assays, epidemiological studies, and statistical modelling. This will be achieved through four aims: (i) Investigate febrile patients in longitudinal studies in Senegal, Cameroon, and Madagascar; (ii) Develop a multiplex serological assay for viral, parasitic, and bacterial pathogens in Africa; (iii) Create statistical and machine learning tools for multiplex data; and (iv) Implement sero-epidemiological analyses of long-term trends in infectious disease in general populations.

The key technological output will be a multiplex serological assay using Luminex bead-based technology to measure antibodies to infectious diseases transmitted in Africa. The assay will test small volume blood samples for antibodies to >300 antigens from >100 pathogens. We will test samples from prospective cohorts of febrile patients to provide an in-depth assessment of the burden of febrile illness, and from 50,000 biobanked samples dating back over 30 years to assess long-term trends in multi-pathogen epidemiology in the general population.

The knowledge generated from this interdisciplinary project will have major implications for how we study, track, prevent and treat febrile illness in Africa.

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2024-COG

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Host institution

INSTITUT PASTEUR
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 150 000,00
Address
RUE DU DOCTEUR ROUX 25-28
75724 Paris
France

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Region
Ile-de-France Ile-de-France Paris
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 150 000,00

Beneficiaries (4)

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