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Immune-Me-on-a-Chip: A Personalized-Immunized-Human In Vitro Platform for Studying Human Physiology and Biological Threats

Project description

A breakthrough in immune system research

Current models for studying the adaptive immune system rely on animal testing or in vitro methods. A major hurdle in creating human-relevant models is that adaptive immune cells attack non-autologous tissue, preventing long-term co-culture. The ERC-funded Immune-Me project will address this challenge with a pioneering organ-on-a-chip platform. The system will integrate six vascularised organ-chips, allowing the integration of immune cells to occur naturally. This platform will provide groundbreaking insights into immune interactions, organ transplantation and responses to infections like antibiotic-resistant E. coli. By advancing personalised medicine, Immune-Me paves the way for a new era in immunological research.

Objective

Understanding how the adaptive immune system works is of monumental scientific importance. It is remarkable, then, that despite the sophisticated modeling technologies available today, there are no human-relevant in vitro platforms mimicking the adaptive immune system in a physiological environment. Rather, state-of-the art models involve animals or in vitro systems that capture isolated elements of the immune response (e.g. activation by tumor cells). The challenge in developing immunized in vitro models is that adaptive immune cells cannot be co-cultured with non-autologous tissue, as they become activated and destroy it. We propose a groundbreaking paradigm that tackles this challenge, while advancing the greater ideal of personalized medicine. The approach builds on my expertise with the Organ-on-a-Chip: a microfluidic platform comprising human tissue that closely mimics organ functionality. I will create a novel platform integrating six vascularized Organ-Chips, all originating from iPSCs derived from a specific individual, which have been differentiated into specific tissue types. This fully isogenic platform will accommodate the donors adaptive immune cells: Because they originate from the same source, they will not be activated. Indeed, preliminary results support this hypothesis. The resultant system, Immune-Me-on-a-Chip, will constitute a first-of-its kind personalized-immunized-human platform for studying human physiology and biological threats. I will use the system to explore fundamental biological questions: (i) understanding how different isogenic and non-isogenic tissues interact with the immune system, e.g. in organ transplantation; and (ii) identifying how pathogens (antibiotic-resistant E. coli), as well as antibiotic treatment, affect human physiology and the immune response. This research will revolutionize the study of human physiology in general and of immunity in particular, and will open the door to a new era of personalized medicine.

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2024-COG

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Host institution

TEL AVIV UNIVERSITY
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 2 999 263,00
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 2 999 263,00

Beneficiaries (1)

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