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The impact of chromatin on genome organisation, function and evolution

Project description

3D genome organisation in insects

DNA in cells is packaged in association with proteins, forming chromatin. During cell division, chromatin condenses to package DNA into chromosomes, which is critical for the accurate distribution of genomic material in daughter cells. Despite its fundamental importance, there is considerable variation in the chromatin composition of eukaryotes. The ERC-funded CHROMOGENEVO project will explore how chromatin composition affects genome architecture in insects. Researchers will investigate how epigenetic changes in chromatin folding lead to unusual centromere patterns, which are key regions for chromosome movement. Moreover, the study will include an evolutionary analysis of the influence of certain 3D genome organisation features on genome function through time.

Objective

Chromatin contributes to many important processes ensuring the proper functioning of eukaryotic cells. In addition, it shapes linear and spatial genome organisation, and specifies chromosomal regions including centromeres that play a key role for chromosome segregation. Chromatin-associated factors also fold the genome in 3D to enable the transfer of chromosomes during mitosis or to regulate gene expression. Chromatin composition is however not identical among eukaryotes. How differences in chromatin composition contribute to different linear and spatial genome organisations is largely unknown.
We aim to address these unknowns in three non-overlapping aims focusing on Lepidoptera and other insects as a model system. First, we will investigate how the disruption of an essential chromatin-based epigenetic loop underlies a drastic case of centromere divergence. To this end, we will perform homology studies on centromere-specifying factors to correlate them with centromere profiles in representative insects that have convergently evolved a holocentric architecture (chromosome-wide centromeres) from monocentric ancestors (centromeres restricted to one chromosomal region). Second, applying a combination of genomics, microscopy and biophysical simulations in the holocentric moth Bombyx mori we will test how the chromosome-wide distribution of centromeres affects the spatial organisation of mitotic chromosomes. Third, using B. mori and exploiting extensive genomic datasets from related Lepidoptera we will investigate how unprecedented features in 3D genome organisation including a new chromatin compartment that we identified affect genome function and organisation over evolutionary time.
With our proposal, we will reveal how changes in chromatin lead to reorganisation of essential genomic regions and shape the topology of chromosomes in 3D, and in turn how this spatial dimension influences genome structure across evolution.

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2024-COG

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Host institution

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 876 250,00
Address
RUE MICHEL ANGE 3
75794 PARIS
France

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Region
Ile-de-France Ile-de-France Paris
Activity type
Research Organisations
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Total cost

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€ 2 000 000,00

Beneficiaries (2)

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