Objective
This project will develop a new method for spatial manipulation of light by designing and validating assemblies of plasmonic nanostructures and fluorophores to advance visualization of densely packed biological structures and their dynamics. The visualization of densely packed biological structures is extremely challenging, yet crucial for understanding dynamic biological processes in virtually any context; light manipulation at the nanoscale using plasmonic nanoparticles has the potential to magnify nanoscopic sample structure while simultaneously enhancing the optical response beyond the capabilities of current super-resolution microscopy techniques. While the ability of metal nanostructures to enhance the response of fluorophores has been utilized for decades, we are still far from understanding the underlying mechanisms of plasmonic enhancement. To fully exploit the potential of this approach, we need to know how spectral overlap between the plasmonic nanoparticle and the absorption and emission channels of the fluorophore affects the coupling efficiency and spatial projection. To translate these effect into applications, we have to understand how the fluorophore-particle distance affects the far-field projection of the fluorophore. We will use self-assembled DNA, single-molecule localization microscopy, and finite difference time domain electromagnetic simulations to measure, describe and reconstruct sub-diffraction limited shifts in the projection of plasmon-coupled fluorophores. This will let us pave the way to the design of a magnifying device aimed at visualizing densely packed biological structures. Fundamental understanding of plasmonic coupling obtained in this project will be directly applicable in various fields of research by providing a tool for high-resolution visualization of molecular structures and their dynamics in the form of plasmonic assemblies, and enabling precise control over enhancement in fluorescence and Raman systems.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences physical sciences optics microscopy super resolution microscopy
- engineering and technology nanotechnology nano-materials
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.4.1 - Widening participation and spreading excellence
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-WIDERA-2023-TALENTS-02
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
182 00 PRAHA
Czechia
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.