Skip to main content
Go to the home page of the European Commission (opens in new window)
English English
CORDIS - EU research results
CORDIS

Stepping into the light: exploring mono-ADP-ribosylation through super resolution STED microscopy

Objective

ADP-ribosylation (ADPr) is a key post-translational modification catalyzed by the ADP-ribosyltransferase (ART) superfamily, including poly-ADP-ribose polymerases (PARPs), which, despite their name, can transfer either a single (mono-ADPr) or multiple (poly-ADPr; PAR) units of ADP-ribose to their targets. This process occurs in response to various biological and pathological stimuli, regulating numerous cellular functions. For many years, research in the ADPr field focused primarily on poly-ADPr PARPs, often overlooking the physiological importance and therapeutic potential of mono-PARPs. Recently, the development of new reagents, including versatile antibodies to detect mono-ADPr, provided essential tools to finally explore this elusive modification. Emerging evidence, partly obtained by using these antibodies, suggests that mono-ADPr responses could be promising therapeutic targets for immune-related diseases, pushing mono-ADPr into the spotlight. However, this growing pharmaceutical interest contrasts with our limited understanding of the molecular mechanisms and biological functions of mono-ADPr and the catalyzing enzymes. In this MSCA project, I aim to shed light on the functions of the underexplored mono-ADP-ribosylation by pioneering the development of super-resolution microscopy methods to visualize mono-ADPr localization across different cellular structures. Through this cutting-edge approach, my research will provide unprecedented insights into the spatial dynamics of mono-ADPr, potentially uncovering novel aspects of its functional roles in cells and providing powerful new methods for the ADP-ribosylation field. Additionally, I will investigate the crosstalk between two PARPs that, according to preliminary data from the host lab, may collaboratively mediate ADPr at the nuclear pore complex, providing insights into the molecular mechanisms underlying the findings from our microscopy studies.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
This project's classification has been human-validated.

Keywords

Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)

Programme(s)

Multi-annual funding programmes that define the EU’s priorities for research and innovation.

Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

See all projects funded under this funding scheme

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) HORIZON-MSCA-2024-PF-01

See all projects funded under this call

Coordinator

UNIVERSITAT ZU KOLN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 202 125,12
Address
ALBERTUS MAGNUS PLATZ
50931 KOLN
Germany

See on map

Region
Nordrhein-Westfalen Köln Köln, Kreisfreie Stadt
Activity type
Higher or Secondary Education Establishments
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

No data
My booklet 0 0