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Unraveling Bilobalide Biosynthesis for Sustainable and Scalable Production in Yeast

Objective

Ginkgo biloba extracts are among the most widely used plant-based health supplements in the United States and Europe. However, increasing demand has led to unsustainable overharvesting, resulting in Ginkgo biloba being classified as an endangered species. These extracts contain bioactive compounds, such as ginkgolides and bilobalide. Bilobalide, in particular, is valued for its neuroprotective, anti-ischemic, and insect-repellent properties, with potential applications in treating Alzheimers disease, dementia, sclerosis, and as a biopesticide.
To address these societal and environmental challenges, this project aims to develop a scalable, sustainable production platform for bilobalide using synthetic biology in bakers yeast. This approach will reduce pressure on G. biloba populations, contributing to biodiversity preservation while promoting innovative biotechnological solutions to meet the demand for natural products. A central focus of this research is to elucidate the biosynthetic pathway of bilobalide, which remains poorly understood.
It is hypothesized that bilobalide is synthesized either via sesquiterpenoid pathways or through the degradation of ginkgolides. Using complementary experimental systems in yeast and tobacco, I will systematically test both hypotheses to uncover the key biosynthetic steps. Once the bilobalide biosynthesis pathway is elucidated, I will engineer it in bakers yeast to create a cost-effective, scalable, and sustainable production system.
This project will leverage the tools and datasets available in the host lab, along with my expertise in plant secondary metabolism and pathway gene characterization, to achieve its goals. It aligns with the European Unions priorities of fostering innovation, sustainability, and natural resource conservation, while advancing biotechnology for health and agriculture. Ultimately, this research will contribute to the green transition and aid in the conservation of endangered species like G. biloba.

Fields of science (EuroSciVoc)

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Coordinator

KOBENHAVNS UNIVERSITET
Net EU contribution
€ 263 393,28
Address
NORREGADE 10
1165 Kobenhavn
Denmark

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Region
Danmark Hovedstaden Byen København
Activity type
Higher or Secondary Education Establishments
Links
Total cost
No data