Project description
Tubulin isotypes: code carriers for structure and function of microtubule networks
Patterned microtubule networks in muscle cells provide structural support, facilitate organelle movement, and help maintain muscle fibre integrity, particularly under mechanical stress. They are essential for muscle development and function. These networks are highly dynamic. Dysfunction of microtubule networks is linked to various muscular dystrophies. With the support of the Marie Skłodowska-Curie Actions programme, the SKM-REFORMAT project aims to investigate whether the tubulin isotypes in the network encode microtubule properties and patterns. Using in vitro tools, animal models, and state of the art microscopy, SKM-REFORMAT will investigate the effects of selected tubulin isotypes on structure and function, from macroscopic phenotypes to molecular mechanisms in health and disease.
Objective
Microtubules are formed by polymerization of tubulin heterodimers. Tubulin monomers rise from 16 genes each with their own spatiotemporal signature, resulting in a patterned microtubule network. As tubulin dimers define binding microtubule associated proteins (MAPs) and occurring post-translational modifications (PTMs), I wonder whether microtubule properties and patterns are encoded/defined by the tubulin isotypes themselves. This proposal aims at 1-confirmation of the expressed tubulin isotypes in skeletal muscle, depending on developmental stages, sex, and fiber type. 2-Understanding of how tubulin isotypes contribute to macroscopic phenotypes such as myoblasts fusion, general fiber organization and muscle contraction. 3-Deciphering of the molecular mechanisms at play upon tubulin isotype loss-of-function. 4-Assessement of potential beneficial effects of tubulin loss-of-function in diseases such as Duchenne muscular Dystrophy (DMD), Centronuclear Myopathy (CNM), and myasthenia. Using innovative in vitro differentiation tools, animal models and state of the art microscopy, I will explore all potential effects of selected tubulin isotypes, from macroscopic phenotypes to molecular mechanisms, as well as in disease. Expected techniques to use are: RT-q-PCR, co-cultures motoneurons-myoblasts, CRISPR/Cas9 mediated KO, shRNA KD, gene transfer by AAV injection, force measurement in vivo and ex vivo, immunohisto- and cytochemistry, live-imaging with fluorescent probes, and image analysis. Microtubule and tubulin being involved in many types of diseases (cancer, development, degeneration…) this project will lead to many economic, social and therapeutic benefits.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
This project's classification has been human-validated.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
This project's classification has been human-validated.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy
- medical and health sciences basic medicine neurology muscular dystrophies duchenne muscular dystrophy
- medical and health sciences basic medicine medical genetics
- natural sciences biological sciences molecular biology
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2024-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
69622 Villeurbanne Cedex
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.