Project description
Nuclear metabolism and epigenetic regulation during implantation
Epigenetic mechanisms can regulate gene expression through chemical modifications of DNA bases and changes to the chromosomal structure without altering DNA sequences. During embryonic development, epigenetic changes play a pivotal role in determining lineage specification. With the support of the Marie Skłodowska-Curie Actions programme, the NucMeta project will focus on the role of metabolism on the epigenetic landscape during early development. Researchers will employ embryonic stem cell-derived trophoblast stem cells and investigate the regulatory role of specific metabolic enzymes in the nucleus. Using transcriptomic, genomic, and epigenomic data, they aim to uncover how nuclear metabolic activity directs gene regulation during embryo implantation.
Objective
Embryogenesis involves dynamic epigenetic and transcriptional changes as the zygote divides and cells differentiate into embryonic (epiblast) and extra-embryonic (primitive endoderm and trophectoderm) lineages. Metabolites serve as cofactors or substrates for chromatin-modifying proteins, thus shaping the global epigenetic landscape. Intriguingly, during early development, multiple metabolic enzymes translocate to the nucleus where they might fuel chromatin modifiers. How the interplay between nuclear metabolism and epigenetics regulates cell fate transitions remains unclear. With this proposal, I aim to unveil the role of nuclear metabolism in regulating lineage-specific phenotypes during early development, using implantation as a model. I will focus on this developmental stage as it is characterised by dynamic metabolic and chromatin rewiring. Since implantation relies on proper trophectoderm specification and maturation, I will use embryonic stem cell-derived trophoblast stem cells that transit between pre- and post-implantation-like states in vitro. Specifically, I will establish novel tools to target the degradation of key metabolic enzymes exclusively in the nucleus and assess the impact on gene expression and chromatin modifications. Moreover, I propose to identify the genomic targets and protein partners of nuclear metabolic enzymes. Ultimately, the integration of transcriptomic, genomic, and epigenomic data will unveil the metabolic determinants underlying gene expression and epigenetic changes at implantation.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences cell biology cell metabolism
- natural sciences biological sciences developmental biology
- medical and health sciences clinical medicine embryology
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- natural sciences biological sciences genetics epigenetics
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2024-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
1165 KOBENHAVN
Denmark
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.