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Unravelling the molecular regulation of microbe-induced plant resistance to root-knot nematodes in tomato

Project description

Beneficial soil microbes protect crops from root-infecting nematodes

Root-knot nematodes are small parasitic worms that pose a serious threat to global food security by damaging a wide range of crops, including tomatoes. Current control strategies have serious limitations and are often environmentally harmful. Beneficial soil microbes offer a promising alternative, as they can prime plant immune systems to resist nematode attacks. This phenomenon is known as microbe-induced plant resistance. With the support of the Marie Skłodowska-Curie Actions programme, the MIR2RKN project is investigating the molecular mechanisms underlying the microbes' protective effect using tomato plants. In this context, researchers will employ multi-omics approaches, computational biology and semi-field experiments. Project results will generate important fundamental knowledge and pave the way towards the development of sustainable crop protection strategies against root-knot nematodes.

Objective

Root knot nematodes (RKNs) represent a major problem for global food security. Current control strategies of RKN have critical limitations. Beneficial root-associated microbes, like rhizobacteria, can enhance the plant immune systems, boosting resistance to a broad spectrum of attackers, including RKNs. This phenomenon, known as Microbe-Induced plant Resistance (MIR), has emerged as a promising biotechnological tool for a sustainable RKN control. However, there are still important challenges in the implementation of MIR-based products in RKN protection programs. We are just starting to uncover molecular mechanisms underlying MIR’s effectiveness to RKN, which have complex, long-term relationship with plants, including well-differentiated infection stages. The main aim of MIR2RNK is to contribute to elucidating molecular mechanisms that regulate MIR functioning to RKN in tomato. MIR2RKN will use a multidisciplinary approach combining multi-omics high-throughput techniques, computational biology, functional genomics, and mesocosm experiments to achieve three main objectives: (1) To evaluate the impact of rhizobacteria on tomato resistance to RKN throughout the infection cycle, and validate the economic viability of these findings under close-to-real field conditions; (2) To uncover key molecular traits of the tomato plant immune system that regulate rhizobacteria-MIR to RKNs across the infection cycle, and (3) To decipher the specific role of tomato polyamine metabolism in rhizobacteria-MIR to RKNs throughout the RKN’s infection cycle. MIR2RKN will enhance our understanding of how MIR is regulated during the dynamic infection cycle of RKNs. This knowledge on how MIR functions, will contribute to developing sustainable crop protection strategies against RKNs. Moreover, MIR2RKN will strongly benefit the applicant, as he will gain independence in research, wider visibility, and will be trained on fundamental skills for proceeding in the next step of his scientific path.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2024-PF-01

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Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Net EU contribution

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€ 209 914,56
Address
CALLE SERRANO 117
28006 MADRID
Spain

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Comunidad de Madrid Comunidad de Madrid Madrid
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Research Organisations
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