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Form for function: regulation of bundle sheath cell architecture for C4 photosynthesis

Project description

Enhancing photosynthesis in crops

To feed the growing global population, we must improve crop productivity. Maize and sorghum are up to 50 % more efficient than other common crops like wheat and rice, because of the photosynthetic pathway they use. Understanding how plants achieve this efficiency could unlock novel strategies for engineering crops. The ERC-funded CELL4 project focuses on the development of specialised sheath cells that provide enhanced photosynthetic efficiency in maize. Researchers will uncover the underlying molecular mechanisms through high-resolution 3D electron microscopy and metabolite and genetic analysis. By revealing how this architecture is established and how each trait contributes to photosynthetic function, the project will inform strategies for engineering crops and maintaining global food security.

Objective

PROPOSAL SUMMARY
The architecture of specialised cell types intricately aligns with cell function. A prime example is the bundle sheath (BS) cells of plants conducting C4 photosynthesis, in which CO2 is concentrated. This concentration mechanism increases photosynthetic efficiency by up to 50% compared to plants that use the more common C3 photosynthetic pathway. BS cells of C4 plants have two key adaptations: increased connectivity with adjacent mesophyll cells via numerous plasmodesmata that facilitate metabolite exchange for building CO2 concentration, and an increased number of organelles which are uniquely positioned at one side of the cell. Despite the proposed functional importance of these traits, it is not understood how they arise during leaf development.

In this project, I aim to elucidate mechanisms governing BS cell architecture. I recently discovered that the polar localisation of plasmodesmata and organelles in BS cells occurs early in leaf development following light exposure in multiple C4 plants. I will use 3D electron microscopy to study BS cell development at high resolution in the C4 grass sorghum and mass spectrometry imaging to map key C4 metabolites, linking structure to metabolic function. I will then investigate molecular mechanisms involved in the development of both traits using mutants that are disrupted in components known to influence plasmodesmata development and organelle positioning in C3 species. I will identify additional components by looking for interaction partners of these components in C4 BS cells. In mutants showing defects in either trait, I will study the impact on metabolite gradients and photosynthetic efficiency, and will explore interdependency between the two polar traits. Overall, my project will reveal mechanisms essential for the development of leaf architecture in C4 plants, and will inform the design strategy for engineering C4 photosynthesis into C3 crops to increase yield.

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(opens in new window) ERC-2025-STG

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Host institution

JOHN INNES CENTRE
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 499 637,00
Address
NORWICH RESEARCH PARK COLNEY
NR4 7UH NORWICH
United Kingdom

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Region
East of England East Anglia Breckland and South Norfolk
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 499 637,00

Beneficiaries (1)

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