Objective
"The remarkable morphological transformation that cells undergo during mitosis has been studied for >140 years, but how mitotic chromosomes are built from interphase nuclei remains unknown and challenging to study. We propose to uncover the mechanisms for this process at high-resolution in cells and in vitro, a task that requires coordinated implementation of innovative experimental designs in several disciplines. We will engineer mutant and hybrid cell lines that enter mitosis with minute-by-minute synchrony, allowing us to develop new “kinetic sectioning” methods to map the intracellular distribution of chromatin near the nuclear envelope, from nucleosomes to mitotic chromosomes at unprecedented resolution, trace the action of individual condensin complexes as they produce loops during mitotic chromosome formation and efficiently isolate mitotic chromosomes for biochemical (4D proteomics) and structural analysis. We will combine genome-wide chromatin mapping, super-resolution microscopy and cryo-ET with innovative image analysis and modelling. In addition to studies of conventional chromosomes, we will study tiny natural ""dot chromosomes"" from chicken cells. Only a few megabases in size, these natural models for their larger counterparts will be isolated for detailed 4D proteomic and structural analysis and used to map scaffold and loop organisation at high resolution. Our team will address mitotic chromosome formation from multiple complementary angles in a synergistic approach where insights from collaborative computational analysis, cross-linking and structural data will inspire and define questions, both predicted and unforeseen. We will discover new insights as condensed mitotic chromosomes are built from active interphase chromatin across a wide range of physical and temporal dimensions. The information and technology that we develop will be generally applicable to other cellular systems, contributing to a paradigm change in structural and cellular biology.
"
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
-
HORIZON.1.1 - European Research Council (ERC)
MAIN PROGRAMME
See all projects funded under this programme
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-ERC-SYG - HORIZON ERC Synergy Grants
See all projects funded under this funding scheme
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2025-SyG
See all projects funded under this callHost institution
Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
8006 Zurich
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.