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MicroSwiss: Affordable Add-On for Polarization, Lifetime, & Super-Resolved Imaging and Tracking

Objective

MicroSwiss aims to develop and validate a plug-and-play microscopy add-on that enhances fluorescence microscopy by combining super-resolution imaging, fluorescence lifetime multiplexing, molecular orientation analysis, and high-speed single-particle tracking into a compact system. This innovative approach overcomes key limitations of current imaging techniques, including hardware complexity, high costs, slow acquisition speeds, and restricted molecular orientation tracking. Addressing these challenges makes high-resolution imaging more accessible to the broader scientific community. The project will first focus on prototype development, integrating a polarization-sensitive prism system, a SPAD-based fluorescence lifetime extraction module, and novel algorithms into a functional imaging system with custom software. This system will enable molecular tracking and fluorescence lifetime analysis, providing researchers with new capabilities to study dynamic biological processes at nanometre resolution. Next, the project will demonstrate the effectiveness and robustness of the add-on across different biological systems and experimental conditions. Benchmarking experiments will evaluate aerolysin pore formation dynamics, molecular diffusion, and orientation-sensitive imaging in varied membrane environments. Planned validation experiments will establish MicroSwiss as a versatile and reliable tool for studying protein assembly and molecular interactions. Beyond technical validation, the project will assess commercialization potential, including market positioning, IP strategy, and business development. Collaboration with EPFL’s Enable program and industry partners will support the transition from prototype to minimum viable product. By the project’s end, MicroSwiss will have reached TRL 5 with a clear roadmap for commercialization through licensing, partnerships, or a dedicated start-up, ensuring its impact in academic research, drug discovery, and nanotechnology applications

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

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HORIZON-ERC-POC - HORIZON ERC Proof of Concept Grants

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2025-POC

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Host institution

ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 150 000,00
Address
BATIMENT CE 3316 STATION 1
1015 LAUSANNE
Switzerland

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Region
Schweiz/Suisse/Svizzera Région lémanique Vaud
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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Beneficiaries (1)

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