Objective
"Fluorescence is a powerful technique that uses molecular tags, each emitting a unique spectral fingerprint of light, to highlight specific cells or proteins. This enables researchers to map cellular interactions and mechanisms. However, as our research needs grow and we use more tags to create a complete picture, their fingerprints inevitably overlap, creating a mixed signal that obscures critical biological information.
Current state-of-the-art unmixing methods operate with a critical restriction: they require the exact number of fluorescent components present and/or the precise spectral fingerprint of each one. This forces time-consuming pre-calibration and prevents the system from identifying unexpected signals, such as tissue autofluorescence, which are often part of the scientific question itself.
The PRISM project will overcome this barrier by developing the first-ever ""double-blind"" unmixing framework, which requires neither the spectra nor the number of components to be known in advance. The core of this innovation is a novel, phasor-guided deep learning architecture that integrates physically-constrained generative models (Transformers, Diffusion Models) to analyze data from spectral microscopy and spectral flow cytometry.
To ensure maximum impact, the project will deliver three key outcomes: 1) A curated, open-access benchmark dataset of synthetic and real-world data; 2) A validated, user-friendly, open-source Python toolkit for the scientific community; and 3) New biological insights generated by applying the tool to map complex immune cell interactions in rheumatoid arthritis models.
This fellowship will be crucial for my transition to an independent research leader. By developing a high-impact technology at the intersection of AI, physics and biology, I will gain the unique skillset to lead a future academic group or deep-tech venture, contributing directly to Europe’s leadership in both life sciences and artificial intelligence."
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2025-PF
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
44139 Dortmund
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.